Boschniakia rossica extract significantly increased the SOD activity, decreased the MDA content, and inhibited the MAO activity in the brain tissue. It was observed under microscope that Boschniakia rossica extract could retrieve the degeneration of mitochondrion
Boschniakia rossica extract can clear the free radicals for D-galactose induced senile rats.
Learning and Memory - To investigate the impact of Boschniakia rossica extract on the content of Ach and learning and memory of the β-amyloid protein(β-AP)-induced model rats. The Traditional Chinese Medicine groups were given orally with three different dose(high,medium and low) of Boschniakia rossica extract.After the treatment,the learning,memory capability and the content of Ach in the hippocampus,and cortical parts of the rats were measured.
Boschniakia rossica extract can enhance the content of Ach and improve the learning and memory capability of AD rats.
Anti-Inflammatory - In this study they investigated the effect of Boschniakia rossica (BR) extract on expression of GST-P, p53 and p21ras proteins in early stage of chemical hepatocarcinogenesis in rats and its anti-inflammatory activities.
Anti-inflammatory activities of BR were studied by xylene and croton oil-induced mouse ear edema, carrageenin, histamine and hot scald-induced rat pow edema, adjuvant-induced rat arthritis and cotton pellet-induced mouse granuloma formation methods.
BR extract exhibited inhibitory effect on formation of preneoplastic hepatic foci in early stage of rat chemical-hepato-carcinogenesis. Both CH2Cl2 and H2O extracts from BR exerted anti-inflammatory effect in rats and mice.
Oxidative Stress - In this study they investigated the intervention effect of aqueous fractions from Boschniakia rossica (BRAF) on hepatic oxidative stress in mice with liver injury induced by carbon tetrachloride (CCl4).
Mice were treated intragastrically with silymarin or BRAF once every day for 7 days. At the end of the experiment, CCl4 was injected intraperitoneally into the mice to establish the acute liver injury model.
BRAF reduces hepatic oxidative stress in mice with acute liver injury induced by CCl4, thereby showing the protective effect on mice with acute liver injury induced by CCl4.
Meanwhile a synergistic effect was observed in boosting various immunity functions when the tumor bearing mice receiving BRP plus 5-FU administration, such as stimulating lymphocytes proliferation, increasing NK cell cytotoxicity.
The results showed that BRP combined with 5-FU presented synergistic effects on antitumor activity in tumor bearing mice. In conclusion, the combination of BRP may boost the suppressed immunity in tumor bearing mice subject to 5-FU chemotherapy, and could serve as a new, promising approach for cancer treatment.
Apoptosis - The aim of this study was to explore the anti-tumor potential of a polysaccharide isolated from Boschniakia rossica (BRP) in Hep2 human larynx squamous carcinoma cells.
The data demonstrates that BRP shows potent anti-tumor activity in human larynx squamous carcinoma, largely through induction of G0/G1 cell cycle arrest and apoptosis. Activation of both mitochondria-mediated and death receptor-mediated apoptosis pathways is involved in the cytotoxicity of BRP.
The BRP was extracted with organic solvent and HR column. We treated Hep2 laryngeal carcinoma cells with different concentrations of BRP, then assessed cell growth inhibition rate by flow cytometry and apoptosis index by TUNEL staining.
Flow cytometry results showed that BRP inhibited Hep2 cell proliferation in a dose-dependent manner (p<0.05), and TUNEL staining indicated that BRP significantly increased Hep2 apoptosis index (p<0.05).
BRP might induce cell apoptosis by regulating the expression level of cell apoptosis-associated proteins, suggesting strong anti-laryngeal cancer activity.