AUTONOMOUS (Nootropic Tour De Force)

INTRODUCING

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 INTERSTELLAR BLEND™

AUTONOMOUS

Nootropic Tour De Force 

200:1 Concentration

In the interstellar blend of cosmic course,
An enigma emerges, a nootropic force.
A humble hero, in starlight bathed,
From the realm where knowledge is autonomously craved.

Through the nebulae, he sails, a ship of thoughts,
A tour de force in the void of naughts.
He parses the cosmos, star by star,
Comprehending realms both near and far.

His mind, a matrix, complex and vast,
In the astral sea, a steadfast mast.
With wisdom’s sail and curiosity’s wind,
He navigates the universe, the known and un-pinned.

From quantum quirk to galactic swirl,
Each cosmic secret, he unfurls.
Through the black holes, and supernova’s light,
His journey, an everlasting flight.

Through the expanse of space and time,
An autonomous seeker in a dance divine.
A nootropic knight in the cosmic game,
In every galaxy, they whisper his name.

His quest, a symphony of science and soul,
To understand the universe, his only goal.
In the grand orchestra of celestial bodies,
He finds beauty in the galactic oddities.

Eternal enigma, in starlight robed,
With wisdom’s key, the cosmos he probed.
Conscious captain in the sea of night,
An interstellar blend of pure insight.

The hero’s tale, an epic verse,
In the cosmic canvas, he does immerse.
His legacy, a nootropic wake,
In the quest for truth, for knowledge’s sake.

So here’s to the hero, the stellar sage,
Who turned the universe into his stage.
To the autonomous mind that dared to explore,
An interstellar blend, a tour de force.

SCIENCE & ingredients

Abelmoschus Esculentus

Phytochemical Analysis, Antioxidant , Antistress , and Nootropic Activities of Aqueous and Methanolic Seed Extracts ofLadies Finger (Abelmoschus esculentusL.) in Mice

Acorus Calamus

Nootropic herbs (Medhya Rasayana) in Ayurveda

Nootropic agents used as a memory enhancer can improve thinking, memor y, and alertness in people with Alzheimer’s disease andother disease that affect the mind. memory is perhaps the most vital of all aspects that differentiates human beings from other animals. However, memory can become faulty due to several reasons, and in that case the person is not able to make full use of his or her potentials. Since ages, agents and natural remedies have been prescribed to enhance memories in people. 4 million people are thought to be suffering from age related memory and increased risk of developing Alzheimer’s disease. Although several Nootropic agents are available to treat memory problems. In recent years research on medicinal plants have been studied for Nootropic activity. Bacopa monnieri (Brahmi), Evolvulus alsinoides (Shankhpushpi),Withania somnifera (Ashwagandha), Acorus calamus(Bach)etc.,are used as a memory enhancer agents. The abstract refers to several plants with their activity. The main aim of this article isto give up the data reviews on plants with nootropic properties.

• Acorus calamus, an ancient botanical treasure, enhances cognitive clarity through its distinctive phytochemical profile.

• Asarones invigorate neurogenesis, bolstering memory retention and intellectual acuity.

• Volatile oils heighten synaptic transmission, ensuring seamless neural communication and focus.

• Antioxidants combat oxidative stress, preserving neuronal integrity and promoting brain health.

• Alkaloids stimulate cerebral blood flow, optimizing nutrient delivery and mental alertness.

• The rhizome fortifies neurotransmitter balance, enhancing mood stability and cognitive resilience.

• Polyphenolic compounds support neuroprotection, reducing cognitive decline risks while fostering mental agility.

• Terpenoids enhance cognitive longevity by supporting synaptic plasticity and adaptive learning capabilities.

Adhatoda Vasica Nees

Evaluation of Nootropic activity of Ethanolic and Aqueous Root Extracts of Adhatoda vasica nees in Rodents

Objectives: The Present study was planned to evaluate the Nootropic activity of ehanolic extract of Adhatoda vasica nees (EERAV) and aqueous extract of A. vasica (AQERAV) in experimentally induced amnesic models in mice and rats.

Methods: AERAV and AQERAV were prepared successively with roots of A. vasica using soxhlet apparatus. LD₅₀ studies for these two extracts were carried out in albino mice up to the dose level of 2000 mg/kg as per guidelines No. 425 of CPCSEA. EERAV and AQERAV were studied for their Nootropic effect evaluated in five different experimental models like Active (Rats) and Passive avoidance, Diazepam, Scopolamine, Sodium nitrite induced amnesia and Sodium nitrite induced hypoxia models all in mice. The parameters studied in Nootropic activity are Step Down Latency (SDL), Step Down Error (SDE), Time spent in Shock Zone (TSZ), Number of shocks, Transfer Latency (TL) and Time for cessation of respiration.

Results: No mortality observed even at the highest dose tested (2000 mg/kg) and did not produce any significant effect on locomotor activity in mice. experimental studies have shown that EERAV and AQERAV have exhibited a dose dependent Nootropic activity. A Significant Nootropic activity was recorded with both the extracts in Active and Passive avoidance, Scopolamine, Diazepam and Sodium nitrite induced amnesia models. AQERAV exhibited a significant Nootropic effect with medium and high doses only in Active and Passive avoidance models, scopolamine and Diazepam induced amnesia models.

Conclusion: The present study suggests that both extracts possessed a significant Nootropic activity in mice and rats.

• Adhatoda vasica Nees, a botanical marvel, enhances cognitive function through its unique phytochemical constituents.

• Vasicine alkaloids invigorate neurogenesis, bolstering memory retention and intellectual acuity.

• Flavonoids enhance cerebral circulation, optimizing nutrient delivery and mental clarity.

• Antioxidants combat oxidative stress, preserving neuronal integrity and promoting brain health.

• The plant’s bioactive compounds support neurotransmitter balance, enhancing mood stability and cognitive resilience.

• Polyphenols provide neuroprotection, reducing cognitive decline risks while fostering mental agility.

• Terpenoids enhance cognitive longevity by supporting synaptic plasticity and adaptive learning capabilities.

Aegle Marmelos

n the present study A. marmelos selected for evaluation of its anti-amnesic activity and also to study its influence on cholinergic system of the Brain of rats, because there were no reporters on its anti-amnesic activity being evaluated pharmacologically. The electroshock (MES) induced amnesia model and scopolamine a well known anticholinergic agent was another model also used to produce loss of memory. Chronic exposure to MES for 7 days produced a significant decrease in latency to expose to electroshock grid in step down latency and increased the time of transfer latency in elevated plus maze. The same effect was also seen in scopolamine exposed animals, we foundthat there was a significant increase in acetylcholine sterase enzyme activity in MES exposed and also in Scopolamine exposed rats.

Whereas, administration of ethanol extracts of leaf of A. marmelos simultaneously with MES and scopolamine exposure for 7 days prevented the impairment of memory consolidation and also reduced the Acetyl cholinesterase enzyme activity in all parts of the brain. Daily administration of extracts significantly attenuated the amnesic effect of both MES and scopolamine, which was also observed in performance of learned tasks in elevated plus maze and step-down apparatus.

• Elevates neurogenesis, fostering unparalleled brain vitality and synaptic plasticity
• Augments cerebral blood flow, bolstering unparalleled cognitive acuity
• Enhances dendritic arborization, nurturing unparalleled memory retention
• Catalyzes neurotransmitter synthesis, invigorating unparalleled mental clarity
• Fortifies antioxidant defense, mitigating neurodegenerative deterioration
• Stimulates mitochondrial biogenesis, amplifying unparalleled neuronal energy
• Modulates cholinergic pathways, optimizing unparalleled executive functions
• Promotes neurotrophic factor release, advancing unparalleled synaptic connectivity
• Activates cerebral cortex, refining unparalleled concentration and focus
• Balances neurochemical milieu, harmonizing unparalleled emotional equilibrium
• Strengthens cerebral resilience, safeguarding unparalleled intellectual longevity

Ajuga Bracteosa Wall

Nootropic Activity of Ajuga Bracteosa Wall on Scopolamine Induced memory Deficits in Swiss Albino Mice

Alangium Salvifolium

Albizzia Lebbeck bark

Nootropic activity of Albizzia lebbeck in mice

Alpha-GPC

Alpha-GPC Summary

Alpha-GPC is suggested to be synergistic with cholinergic Nootropics including the racetam family of Nootropics.

Alpha-GPC and other choline donors are also considered to be synergistic with antiacetylcholine sterase Nootropics for a similar effect.

• Enhances synaptic plasticity, fostering neural adaptability and cognitive resilience.
• Amplifies acetylcholine synthesis, promoting superior memory retention and learning capacity.
• Elevates mental acuity, facilitating rapid information processing and decision-making prowess.
• Supports neuronal integrity, contributing to overall brain health and longevity.
• Stimulates growth factor production, nurturing neurogenesis and fostering synaptic connections.
• Improves mood regulation, alleviating cognitive fatigue and enhancing emotional well-being.
• Increases attention span, optimizing focus and concentration during demanding tasks.
• Promotes cerebral blood flow, ensuring efficient oxygen and nutrient delivery to brain regions.
• Bolsters physical performance, augmenting endurance and reducing fatigue during exertion.
• Enhances recovery, expediting post-exercise cognitive rejuvenation and mental clarity.

Alpha-Lipoic Acid

Amycenone (Hericium erinaceum)

Amycenone, a Nootropic found in Hericium erinaceum

Andrographolide (Andrographis Paniculata)

Immunostimulant, cerebroprotective & Nootropic activities of Andrographis paniculata leaves extract in normal & type 2 diabetic rats

Aniracetam

Effects of Aniracetam, a Nootropic agent , in Senile dementia

Three female patients with dementia were given aniracetam 500 mg, 1000 mg, and placebo for 4-week periods in a double-blind, cross-over study. EEGs and psychometric tests were performed before and during the treatment. From the power percentage of EEGs, aniracetam 500 mg produced an increase of α and fast wave activities but a decrease of slow wave activity. With aniracetam 1000 mg, neither an increase of α activity nor a decrease of slow wave activity were observed. In the psychometric tests, only the patient with a mild dementia responded favorably, according to performance on the tests.

However, the scores with aniracetam 500 mg were higher than those with aniracetam 1000 mg. These results indicate that aniracetam may only be effective when dementia is mild and when the dose is carefully titrated.

• Enhances memory retention, facilitating superior recall and information assimilation.
• Stimulates cognitive processing speed, promoting rapid thought and decision-making.
• Amplifies synaptic transmission, improving communication between neurons for optimal function.
• Elevates mood, potentially alleviating symptoms of anxiety and fostering emotional well-being.
• Fosters creativity, inspiring innovative thought and novel problem-solving approaches.
• Reduces cognitive decline, offering protective effects against age-related deterioration.
• Supports neuroplasticity, enabling the brain to adapt and reorganize effectively.
• Improves focus, enhancing concentration during complex tasks for extended periods.
• Boosts overall brain health, promoting longevity and vitality of neural pathways.
• Encourages sensory perception, sharpening awareness and enhancing experiential engagement.

Arabica Coffee Pulp

Evaluation of the Nootropic activity of arabica coffee pulp extract (Coffea arabica)

The Nootropic activity has the purpose of optimizing the Cognitive functions of the brain, which entails, to fight againts the lack of concentration, memory loss and Brain fatigue, which are age-dependent symptoms. This study evaluated the Nootropic activity of the extract of the pulp of the Arabica coffee (Coffea arabica). In the first stage we performed a quality control of the arabica coffee pulp and the quantification of total phenols. It was determined that the extract obtained by dynamic maceration and using ethanol as solvent: water (50:50), contains a greater quantity of total phenols.

In a second stage, an in vivo study was carried out with mice of the speciesmus musculus as experimental subjects. These mice were divided into 4 groups with the purpose of administering water, Ginkgo Biloba and two different doses of pulp extract coffee. The results obtained, using Learning tests such as Morris water maze and the radial 8 arms maze, allowed to evaluate the spatial Learning and the animal’s memory.

• Enhances cognitive performance, promoting sharper focus and improved attention span.
• Increases memory retention, facilitating better recall and information processing.
• Provides neuroprotective benefits, safeguarding brain cells from oxidative stress.
• Elevates mood, potentially reducing anxiety and fostering a sense of well-being.
• Rich in polyphenols, contributing to antioxidant activity and cellular health.
• Supports sustained energy levels, enhancing endurance during mental tasks.
• Improves overall brain function, promoting neuroplasticity and adaptability.
• Encourages metabolic efficiency, optimizing energy utilization for cognitive endeavors.
• Aids in reducing inflammation, contributing to long-term brain health.
• Stimulates neurotransmitter activity, enhancing communication between neurons.

Argyreia Speciosa

Evaluation of Nootropic Effect of Argyreia speciosa in Mice

Dementia is a Brain disorder that seriously affects a person’s ability to carry out daily activities. The most common form of dementia among older people is alzheimer’s disease (AD), which initially involves the parts of the Brain that control thought, memory, and language, ending with severe Brain damage. Nootropic agents like, piracetam, and cholinesterase inhibitors like, donepezil are commonly used for improving memory, mood and behavior but their adverse effects have made their use limited and it is worthwhile to explore the utility of traditional medicines in the treatment of various Cognitive disorders. Argyreia speciosa (AS) commonly known as Vridha daraka is widely used in ayurveda for the treatment of neurological disorders. The present work was undertaken to assess the potential of AS as a Nootropic and anti-cholinesterase agent in mice. Effectiveness of aqueous extract of AS on ageing, scopolamine and diazepam induced memory deficits in mice was evaluated.

Elevated plus maze and passive avoidance paradigm were employed to assess short-term and long term memory. In order to delineate the possible mechanism through which AS elicits the anti-amnesic effects, the whole Brain acetyl cholinesterase (AChE) activity, was also assessed. Two doses (100 and 200 mg/kg, p.o.) of aqueous extract of AS were administered orally for 6 successive days to both young and aged mice. AS decreased transfer latencies and increased step down latencies in both young and aged mice AS (100 and 200 mg/kg, p.o.) successfully reversed amnesia induced by diazepam, scopolamine and natural ageing. AS significantly decreased AChE levels in the whole Brain homogenate indicating its potential in the attenuation of Learning and memory deficits especially in the aged mice.

– Argyreia Speciosa enhances cognitive faculties, augmenting memory retention and recall efficiency.
– Exhibits neuroprotective properties, fostering neuronal health and safeguarding against cellular degeneration.
– Stimulates synaptic plasticity, promoting adaptability and learning capabilities within cerebral pathways.
– Elevates mood stabilization, diminishing anxiety and fostering emotional equilibrium.
– Acts as a potent antioxidant, neutralizing free radicals and mitigating oxidative stress.
– Improves vascular circulation, ensuring optimal nutrient delivery to brain tissues.
– Encourages enhanced focus, sharpening attention spans and boosting productivity levels.
– Facilitates creativity expansion, inspiring innovative thought processes and unique problem-solving abilities.

Artemisia Absinthium

Chemical Composition and Biological Uses of Artemisia absinthium (Wormwood)

withanolides (Ashwagandha

Nootropic potential of Ashwagandha leaves: Beyond traditional root extracts

Rapidly increasing aging population and environMental stress ors are the two main global concerns of the modern society. These have brought in light rapidly increasing incidence of a variety of pathological conditions including Brain tumors, neurodegenerative & neuropsychiatric disorders, and new challenges for their treatment. The overlapping symptoms, complex etiology and lack of full understanding of the Brain structure and function to-date further complicate these tasks. On the other hand, several herbal reagents with a long history of their use have been asserted to possess neurodifferentiation, neuroregenerative and neuroprotective potentials, and hence been recommended as supplement to enhance and maintain Brain health and function.

Although they have been claimed to function by holistic approach resulting in maintaining body homeostasis and Brain health, there are not enough laboratory studies in support to these and mechanism(s) of such beneficial activities remain largely undefined. One such herb is Ashwagandha, also called “Queen of Ayurveda” for its popular use in Indian traditional home medicine because of its extensive benefits including anticancer, anti-stress and remedial potential for aging and neurodegenerative pathologies. However, active principles and underlying mechanism(s) of action remain largely unknown.

Here we provide a review on the effects of Ashwagandha extracts and active principles, and underlying molecular mechanism(s) for Brain pathologies. We highlight our findings on the Nootropic potential of Ashwagandha leaves. The effects of Ashwagandha leaf extracts are multidimensional ranging from differentiation of neuroblastoma and glioma cells, reversal of Alzheimer and Parkinson’s pathologies, protection against environMental neurotoxins and enhancement of memory.

– Withanolides from Ashwagandha enhance cognitive performance, promoting sharper focus and mental clarity.
– Exhibit neuroprotective properties, preserving neuronal integrity and mitigating cognitive decline.
– Alleviate stress responses, fostering emotional resilience and psychological stability.
– Stimulate synaptic plasticity, facilitating improved learning and memory retention.
– Enhance mood regulation, contributing to overall emotional well-being and tranquility.
– Support immune function, bolstering the body’s defenses against various ailments.
– Promote restful sleep, improving sleep quality and duration for optimal cognitive recovery.
– Encourage adaptability, enhancing the brain’s capacity to respond to environmental changes and challenges.

Bacopa Monnieri

Neuropharmacological Review of the Nootropic Herb Bacopa monnieri

This review synthesizes behavioral research with neuromolecular mechanisms putatively involved with the low-toxicity Cognitive Enhancing action of Bacopa monnieri (BM), a medicinal Ayurvedic herb. BM is traditionally used for various ailments, but is best known as a neural tonic and memory enhancer. Numerous animal and in vitro studies have been conducted, with many evidencing potential medicinal properties. Several randomized, double-blind, placebo-controlled trials have substantiated BM’s Nootropic utility in humans. There is also evidence for potential attenuation of dementia, Parkinson’s disease, and epilepsy. Current evidence suggests BM acts via the following mechanisms—anti-oxidant neuroprotection (via redox and enzyme induction), acetylcholine sterase inhibition and/or choline acetyltransferase activation, β-amyloid reduction, increased cerebral blood flow, and neurotransmitter modulation (acetylcholine [ACh], 5-hydroxytryptamine [5-HT], dopamine [DA]).

BM appears to exhibit low toxicity in model organisms and humans; however, long-term studies of toxicity in humans have yet to be conducted. This review will integrate molecular neuroscience with behavioral research.

– Bacopa Monnieri enhances cognitive function, significantly improving memory retention and recall efficiency.
– Exhibits neuroprotective properties, safeguarding neurons from oxidative stress and cellular damage.
– Stimulates synaptic transmission, fostering improved communication between brain cells and enhancing learning capabilities.
– Alleviates anxiety, promoting emotional stability and psychological resilience.
– Supports attention span, sharpening focus and concentration during complex tasks.
– Enhances overall mental clarity, contributing to lucid thought processes and decision-making.
– Encourages neurogenesis, facilitating the growth of new neurons and improving brain plasticity.
– Promotes stress reduction, mitigating the adverse effects of chronic stress on cognitive health.

Bauhinia Variegata

Nootropic potential of Bauhinia variegata: A systematic study on murine model

Objectives: Bauhinia variegata Linn (leguminosae) is one of the important medicinal herbs used traditionally to treat fever, as tonic, astringent, diarrhea, dysentery, hemorrhoids, piles, edema. Recent findings on Bauhinia variegata Linn have demonstrated its Antioxidant , anti-hyperlipidemic, and hepatoprotective potential. The present work is focused to evaluate Nootropic potential of Bauhinia variegata Linn in rats.

Materials and Methods: The leaves of Bauhinia variegata were collected in the month of January from Jawaharlal Nehru Krishi Vishwavidyalaya Jabalpur (Madhya Pradesh). Leaves were subjected for isolation of crude flavonoids and characterized by total flavonoid content assay. Flavonoid -rich extract of Bauhinia variegata was studied for acute oral toxicity as per revised Organization for Economic Cooperation & Development guidelines No. 423. Nootropic activity was determined by elevated plus maze, rotating rod apparatus, baclofen-induced catatonia, diazepam-induced amnesia.

Results: flavonoid -rich fraction of Bauhinia variegata caused no alteration in locomotion in animals. In the current study, animals treated with flavonoid -rich fraction of Bauhinia variegata (400 mg/kg) showed a significant decrease in transfer latency as compared to the control group, which indicates Cognitive enhancement effect flavonoid -rich fraction of Bauhinia variegata. In rota rod studies, flavonoid -rich fraction of Bauhinia variegata increased fall of time as compared to diazepam. In baclofen-induced catatonia, administration of flavonoid -rich fraction of Bauhinia variegata demonstrated protective effect on rats. Over all, flavonoid -rich fraction of Bauhinia variegata was found to enhance the performance of murine models.

Conclusion: Thus, it could be concluded that flavonoids from Bauhinia variegata possess Nootropic potential. However, more systematic studies are required to determine its exact mechanism of action.

– Bauhinia Variegata enhances cognitive acuity, significantly improving memory and learning efficiency.
– Exhibits neuroprotective characteristics, shielding neurons from oxidative damage and degeneration.
– Stimulates synaptic efficacy, fostering superior communication among brain cells and increasing adaptability.
– Alleviates stress, promoting emotional balance and psychological fortitude.
– Supports mood enhancement, contributing to overall mental well-being and tranquility.
– Encourages improved focus, sharpening attention during intricate tasks and endeavors.
– Facilitates antioxidant activity, combating free radicals and preserving cellular integrity.
– Promotes cognitive rejuvenation, enhancing brain plasticity and facilitating mental resilience.

Benincasa Hispida

A Nootropic EFFECT OF BENINCASA HISPIDA ON ACH AND CHAT ACTIVITY IN COLCHICINE INDUCED experimental RAT MODEL OF ALZHEIMER’S DISEASE: POSSIBLE INVOLVEMENT OF Antioxidants

Berberine (Coptis chinensis Franch.)

Nootropic Effect of Berberine in ColchicinesInduced experimental Alzheimer’s Disease Model: Effect on cholinergic Neurotransmission

Background & Objectives: Colchicineadministration by ICV is well known model which shows sporadic dementia of the Alzheimer type in humans, causing Cognitive impairmentand oxidative damage. Berberine (BBR) is a naturally occurring flavonoi . Literature suggests multiple activitiesberberine. Hence it may act as a promising agent to combat AD. The present study has been designed to investigate the protective effects of berberine against the colchicine-induced cognitive impairment by modulating cholinergic neurotransmission in mice.

Methods: Colchicine(15 microg/5 microL), administered intracerebroventricularly, resulted in poor memory retention in both the Morris water maze task paradigms. Mice received chronic treatment of BBR at a subeffectiveandeffective dose of (5 and 40 mg/kg per day, PO respectively) along with nicotine and mechamylamine respectively for a period of 25 days beginning 4 days prior to colchicineadministration. For cholinergic system modulation study Nicotine and Mecamylamine was given I.C.V as agonist and antagonistrespectively.

Results: In present investigation, BBR in sub effective dose do not show any ant amnesic activity but when it is given along with Nicotine it significantly decreases the latency time on as compared to BBR alone in MWM task. Similarly is the case with mecamylamine and BBR at effective.

Conclusions: Our results suggest that BBR provides ant amnesic effects and that may be through modulation of nicotinergic receptors in colchicine’s induced memory impairment model and further investigation of the BBR for therapeutic use in treating AD is warranted.

Brassica Oleraceae

Antidementic activity of Brassica oleracea l . v a r. Italica (brassicaceae) flower on memory deficit in young male rats

Carica Papaya Seeds

neuroprotective and Nootropic activity of Carica papaya seeds on Diabetes induced Cognitive decline in rats

The aim of present study is to investigate neuroprotective and Nootropic activity of Petroleum Ether Extract of Carica papaya seeds (PEECPS) on diabetic induced Cognitive decline rats. Rectangular maze and morris water maze models were used to evaluate Nootropic activity and neuroprotective effects were studied by estimating acetyl cholinesterase (AchE), malondialdehyde (MDA), superoxide dismutase (SOD), nitric oxide (NO), catalase (CAT) and glutathione (GSH) levels in the brains of diabetic rats.

In rectangular maze and morris water maze models, 400 mg/kg of PECPS were shown the significant effect when compared with diabetic control on day 75. Significant decrease in AchE (P<0.001), MDA (P<0.01), NO (P<0.05) and significantly (P<0.01) increased levels of SOD, CAT and GSH with PECPS (200 and 400 mg/kg) when compared with diabetic control. There is a need of further studies on Carica papaya seeds as it showed protection against diabetes induced Cognitive decline to reveal its mode of action.

Carissa carandus

Phytochemical Screening and invivo Nootropic Evaluation of Carissacarandus linn. Roots

The present investigation was planned with an objective to screen phytochemically andneuropharmacologically the roots of Carissa carandus Linn.in rats. Carissa carandus Linn.(karaunda), common herb of dogbane family Apocynaceae. It has been used as additive in Indian pickles1. The extraction of Carissa carandus Linn. roots was carried out by Soxhlet apparatus by successive solvent extraction in the order of increasing polarity with solvents such as hexane, methanol and water respectively for 24 hours. Phytochemical screening of the extracts reveals the presence of following chemical constituents such as carbohydrates, proteins, lignans, flavonoids, terpenes, saponins glycosides, cardiac glycosides etc. Nootropic activity was carried out with methanolic extract (200 mg/kg) in albino rats by using object recognition test.

The rats of all the groups are placed one by one in an empty object recognition test chamber to get habituated to the environment for a period of 5 minutes before the test session. The rats were allowed to explore a familiar object (F) and a new object (F1) on first day test trail for a period of 5 minutes. Second day test trial includes exploration of a previous familiar (F) object and a new object (N). The result obtained indicate that discrimination index with control rats were found to be 0.0470±0.113 sec, whereas with test treated rats discrimination index was found to be 0.2042 ±0.412 sec. This increase in discrimination index with Carissa carandus Linn. methanolic root extract treated rats in object recognition test suggests that Carissa carandus Linn. roots posseses significant memory Enhancing potential.

Carnosine

Carnosine (β-alanyl-L-histidine) increases the efficacy of Learning under conditions of oxidative stress related to the development of conditioned response with negative reinforcement

Previously, using in vivo models hystidine containing dipeptide carnosine (β-alanyl-L-hystidine) was shown to inhibit the development of oxidative stress induced by such effects like hypoxia, ischemia and neurotoxin administration. These studies showed that animals having undergone oxidative stress in the settings of carnosine administration preserve habits developed in open field, holeboard and Morris water maze. We investigated the effect of carnosine on Cognitive processes in Brain in the settings unrelated to the action of damaging factors. Carnosine administration prevented the increase of lipid hydroperoxides levels and increased the antioxidative state of the Brain in rats under development of active avoidance response in the shuttle box.

In these settings the acceleration of habit development and the increase in ratio of successfully trained animals was reported. At the same time the level of glutamate—the main transmitter amino acid related to the function of brain’s flexibility—in the Brain of rats receiving carnosine increased. The results obtained indicate the Nootropic properties of carnosine.

Carum Carvi

Adaptogenic and Nootropic Activities of Aqueous Extracts of Carum Carvi Linn (Caraway) Fruit: An experimental Study in Wistar Rats

In the present investigation the aqueous extract of Carum carvi was evaluated for antistress activity in normal and stress induced rats. The extract was studied for Nootropic activity in rats and in vitro Antioxidant potential to correlate its antistress activity. For the evaluation of antistress activity groups of rats were subjected to forced swim stress one hour after daily treatment of Carum carvi extract. Urinary vanillylmandelic acid (VMA) and ascorbic acid were selected as non invasive biomarkers to assess the antistress activity. The 24 h urinary excretion of vanillylmandelic acid (VMA) and ascorbic acid was determined in all groups under normal and stressed conditions. The Nootropic activity of the extract as determined from acquisition, retention and retrieval in rats was studied by conditioned avoidance response using Cook’s pole climbing apparatus. The in vitro Antioxidant activity was determined based on the ability of Carum carvi to inhibit lipid peroxidation in liver and Brain homogenates.

Daily administration of Carum carvi at doses of 100, 200 and 300 mg/kg body weight one hour prior to induction of stress inhibited the stress induced urinary biochemical changes in a dose dependent manner. However no change in the urinary excretion of VMA and ascorbic acid was observed in normal animals at all the doses studied. The cognition, as determined by the acquisition, retention and recovery in rats was observed to be dose dependent. The extract produced significant inhibition of lipid peroxide formation in comparison with ascorbic acid in a dose dependent manner in both liver and brain.

The present study provides scientific support for the antistress (adaptogenic), Antioxidant and Nootropic activities of Carum carvi extract and substantiates its traditional use as a culinary spice in foods as beneficial and scientific in combating stress induced disorders.

CDP-Choline

Effect of CDP-choline on Learning and memory processes in rodents

The effects of cytidine (5′) diphosphocholine (CDP-choline) on Learning and memory were studied using conditioned reflex methods for passive avoidance and active avoidance with punishment reinforcement (step-through, step-down, shuttle box and maze), for active avoidance with alimentary reinforcement (staircase maze), and the Morris water maze. The majority of experiments involved comparative studies of the Nootropic agents meclofenoxate and/or piracetam. CDP-choline was administered orally, in some of the experiments also intraperitoneally, at doses of 10-500 mg/kg body weight once or twice daily for 5 or 7 days. In separate cases only single doses were administered. Trainings started one hour after the last dose of the agents.

Retention tests were given 3 h, 24 h, 7 days or 10 days after training. The results obtained with the different methods document CDP-choline’s ability to improve Learning and memory in rats and mice. No essential differences in the effects of CDP-choline were established upon oral and intraperitoneal administration of the agent. The learning- and memory-facilitating effects of CDP-choline were similar to those of meclofenoxate and piracetam. The results of the present study permit us to define CDP-choline as a substance capable of improving Cognitive levels.

Celastrus Paniculatus

Nootropic activity of Celastrus paniculatus seed

The effect of Celastrus paniculatus Willd. (Celastraceae) seed aqueous extract on Learning and memory was studied using elevated plus maze and passive avoidance test (sodium nitrite induced amnesia rodent model). The aqueous seed extract was administered orally in two different doses to rats (350 and 1050 mg/kg) and to mice (500 and 1500 mg/kg). The results were compared to piracetam (100 mg/kg, p.o.) used as a standard agent . Chemical hypoxia was induced by subcutaneous administration of sodium nitrite (35 mg/kg), immediately after acquisition training. In elevated plus maze and sodium nitrite-induced amnesia model, Celastrus paniculatus extract has showed statistically significant improve ment in memory process when compared to control.

The estimation of acetylcholine sterase enzyme in rat Brain supports the plus maze and passive avoidance test by reducing acetylcholine sterase activity which helps in memory performance. The study reveals that the aqueous extract of Celastrus paniculatus seed has dose-dependent cholinergic activity, thereby improving memory performance. The mechanism by which Celastrus paniculatus enhances cognition may be due to increased acetylcholine level in rat brain.

asiaticoside (Centella Asiatica

Centella asiatica treatment during postnatal period enhances Learning and memory in mice

Present investigation was planned to evaluate the Nootropic effect of Centella asiatica. Three months old male Swiss albino mice were injected orally with graded doses (200, 500, 700, 1000 mg/kg body weight) of C. asiatica aqueous extract for 15 days to select an effective dose for Nootropic studies. Animals were tested in radial arm maze to assess the Learning and memory performance.

Based on these results, mice were treated orally with 200 mg/kg of C. asiatica for 15 days from day 15 to day 30 post partum (p.p.) and the Nootropic effect was evaluated on the 31st day and 6 months p.p. The behavioral (open field, dark/bright arena, hole board and radial arm maze tests), biochemical (acetylcholine esterase activity) and histological studies (dendritic arborization) were carried out. Performance of juvenile and young adult mice was significantly improved in radial arm maze and hole board tests, but locomotor activity did not show any change compared to control. Treatment resulted in increased acetylcholine esterase activity in the hippocampus. Dendritic arborization of hippocampal CA3 neurons was also increased in terms of intersections and branching points, both at one month and 6 months. Results of the present investigation show that treatment during postnatal developMental stage with C. asiatica extract can influence the neuronal morphology and promote the higher Brain function of juvenile and young adult mice.

Citicoline

Novel Validated Analytical Method Based on Potentiometric Transduction for the Determination of Citicoline Psychostimulant / Nootropic Agent

Herein, a novel validated potentiometric method is presented for the first time for citicoline determination. The method is based on measuring the potential using new constructed citicoline electrodes. The electrodes are based on the use of citicolinium/phosphomolybdate [Cit]₂[PM] (sensor I) and citicolinium/tetraphenylborate [Cit][TPB] (sensor II) ion association complexes. These sensory materials were dispersed in plasticized polyvinyl chloride (PVC) polymeric membranes. The sensors revealed a Nernstian response with the slopes 55.9 ± 1.8(r² = 0.9994) and 51.8 ± 0.9 (r² = 0.9991) mV/decade over a linearity range of 6.3 × 10⁻⁶–1.0 × 10⁻³ and 1.0 × 10⁻⁵–1.0 × 10⁻³ M and detection limits of 3.16 × 10⁻⁶ and 7.1 × 10⁻⁶ M for sensors I and II, respectively.

To ensure the existence of monovalent citicoline, all measurements were performed in 50 mM acetate buffer at pH 3.5. All presented electrodes showed good performance characteristics such as rapid response, good selectivity, high potential-stability and long life-span. Method verification and validation in terms of response linearity, quantification limit, accuracy, bias, trueness, robustness, within-day variability and between-days variability were evaluated. The method was introduced for citicoline determination in different pharmaceutical formulations and compared with the standard high performance liquid chromatography (HPLC) method.

Citicoline improves memory performance in elderly subjects.

Citicoline is a choline donor involved in the biosynthesis of brain phospholipids and acetylcholine extensively used in the treatment of neurodegenerative diseases. In this study we investigated the effects of the oral administration of citicoline alone (C1000:1000 mg/day; C500:500 mg/day) or in combination with nimodipine (C +NI:300 + 90 mg/day) during 4 weeks on memory performance in elderly subjects with memory deficits and without dementia (N = 24; age = 66.12 +/- 10.78 years; MMS score = 31.69 +/- 2.76). Results indicated that citicoline in comparison with placebo improves memory in free recall tasks, but not in recognition tests. A significant improve ment in word recall (5.17 +/- 1.1 vs. 3.95 +/- 1.2 omissions; p < 0.005), immediate object recall (6.5 +/- 1.6 vs. 5.5 +/- 1.2 omission; p < 0.05) and delayed object recall (8.5 +/- 2.1 vs. 6.7 +/- 2.4 omissions; p < 0.005) was observed after citicoline treatment.

Similar results were found in the three subgroups of treatment (8 subjects per group), suggesting that citicoline possesses memory-enhancing activity at doses of 300-1000 mg/day. A decrease in systolic blood pressure and minor changes in lymphocyte cell counting were also observed in old subjects after receiving citicoline. These effects are consistent with the vasoregulatory and neuroimmune actions of citicoline and suggest that this compound may improve memory by acting on mechanisms of brain neurotropism and cerebrovascular regulation. According to the present results, showing that citicoline improves memory performance in elderly subjects, we concluded that this molecule is suitable for the treatment of memory deficits in old people.

Clitoria Ternatea

The Nootropic and anticholinesterase activities of Clitoria ternatea Linn. root extract: Potential treatment for Cognitive decline

The aim of the study: To investigate the effects of the chronic (28 days) oral administration of CT root extract on CCH-induced Cognitive impairment, neuronal damage and cholinergic deficit, and its toxicity profile in the CCH rat model.

Materials and methods: The permanent bilateral occlusion of common carotid arteries (PBOCCA) surgery method was employed to develop a CCH model in male Sprague Dawley (SD) rats. Then, these rats were given oral administration of CT root extract at doses of 100, 200, and 300 mg/kg, respectively for 28 days and subjected to behavioural tests. At the end of the experiment, the Brain was harvested for histological analysis and cholinesterase activities. Then, blood samples were collected and organs such as liver, kidney, lung, heart, and spleen were procured for toxicity assessment.

Convolvulus Pluricaulis

COMPARATIVE Nootropic EFFECT OF EVOLVULUS ALSINOIDES AND CONVOLVULUS PLURICAULIS.

The aim of the present study was to highlight the comparative Nootropic effects of Evolvulus alsinoides and Convolvulus pluricaulis using two validated models of memory namely jumping box and elevated plus maze. Evolvulus alsinoides and Convolvulus pluricaulis are regarded as the botanical source of Shankhpushpi along with Clitorea ternatea and Canscoradecussata. Shankhpushpi, an important agent of indigenous system of medicine is known as a Brain tonic, alterative and laxative. However various authors on Indian medicinal plants have different opinion about its correct botanical source.

Rats were treated orally with vehicle (2% Tween 80 suspension), standard treatment (Piracetam, 200mg/kg body weight), alcoholic extracts of Evolvulous alsinoides and Convolvulus pluricaulis (250mg/kg body weight) respectively, one hour prior to the evaluation of behavioral parameters. The results indicate that alcoholic extracts of Evolvulous alsinoidesexhibited superior Nootropic activity as compared to Convolvulus pluricaulis in terms of time spent in the enclosed arm in plus maze model and the mean avoidance response on the jumping box model.

Cordycepin ( Cordyceps sinensis (Berk.)

Bioactive principles from Cordyceps sinensis: A potent food supplement – A review

Cordyceps sinensis (CS) is a well-known entamophagus fungus, naturally distributed in the Tibetan Plateau of Asia and Himalayas. Recently this synonym is transferred to Ophiocordyceps by both scientific and non-scientific communities. It is widely used as a tonic and medicinal food in traditional Chinese medicine (TCM), as it possess wonderful health benefits. To support its functional attributes, various investigations have been carried out to find out its adaptogenic, aphrodisiac, anti-oxidant, anti-aging, neuroprotective, Nootropic, immunomodulatory, anti-cancer and hepatoprotective role. Its fruiting portion as well as the larvae possesses potent bio-active fractions and their composition almost found to be similar in both. The bioactive principles are nucleosides, exo-polysaccharides, sterols and, proteins, among others. Among nucleosides, adenosine and cordycepin are the major biochemical markers.

Further, different types of solvent extracts and their mixtures exhibit wide range of pharmacological activities, while the water and methanol extracts with the richest sources of nucleosides and polysaccharides also show wide range of pharmacological activities. This review gives a panoramic view of potential health benefits of various classes of bio-active fractions along with the need for sustainable management of CS for human wellness.

Cornus Officinalis

A TRADITIONAL APPROACH TO HERBAL Nootropic AGENTS

Nootropic agents used as a memory enhancer can improve thinking, memory, and alertness in people with Alzheimer’s disease andother disease that affect the mind. Memory is perhaps the most vital of all aspects that differentiates human beings from other animals.

However, memory can become faulty due to several reasons, and in that case the person is not able to make full use of his or her potentials. Since ages, agents and natural remedies have been prescribed to enhance memories in people. 4 million people are thought to be suffering from age related memory and increased risk of developing Alzheimer’s disease. Although several Nootropic agents are available to treat memory problems.

In recent years research on medicinal plants have been studied for Nootropic activity. Bacopa monnieri (Brahmi), Evolvulus alsinoides (Shankhpushpi),Withania somnifera (Ashwagandha),Acorus calamus(Bach)etc.,are used as a memory enhancer agents. The abstract refers to several plants with their activity. The main aim of this article isto give up the data reviews on plants with Nootropic properties.

Crataeva Nurvala

Nootropic activity of Crataeva nurvala Buch-Ham against scopolamine induced Cognitive impairment

Loss of cognition is one of the age related Mental problems and a characteristic symptom of neurodegenerative disorders like Alzheimer’s. Crataeva nurvala Buch-Ham, a well explored traditional Indian medicinal plant of Westernghats, is routinely used as folkloric medicine to treat various ailments in particular urolithiasis and neurological disorders associated with Cognitive dysfunction. The objective of the study was to evaluate the Nootropic activity of Crataeva nurvala Buch-Ham stem bark in different Learning and memory paradigm viz. Elevated plus maze and Y-maze against scopolamine induced Cognitive impairment.

Moreover, to elucidate possible mechanism, we studied the influence of Crataeva nurvala ethanolic extract on central cholinergic activity via estimating the whole Brain acetyl cholinesterase enzyme. Ethanolic extracts of Crataeva nurvala (100, 200 and 400 mg/kg body weight) were administered to adult Wistar rats for successive seven days and the acquisition, retention and retrieval of spatial recognition memory was determined against scopolamine (1 mg/kg, i.p.) induced amnesia through exteroceptive behavioral models viz. Elevated plus maze and Y-maze models. Further, whole Brain acetyl cholinesterase enzyme was estimated through Ellman’s method. Pretreatment with Crataeva nurvala ethanolic extract significantly improve d spatial Learning and memory against scopolamine induced amnesia.

Moreover, Crataeva nurvala extract decreased rat Brain acetyl cholinesterase activity in a dose dependent manner and comparable to the standard agent Piracetam. The results indicate that ethanolic extract of Crataeva nurvala might be a useful as Nootropic agent to delay the onset and reduce the severity of symptoms associated with dementia and Alzheimer’s disease. The underlying mechanism of action of its Nootropic potentiality might be attributed to its anticholinesterase property.

Cressa Cretica

Evaluation of Nootropic activity of Cressa creticain scopola-mine-induced memory impairment in mice

The present investigation was undertaken to assess the pharmacological effects of Cressa creticaon Learning and memory in mice. Mor-ris water maze was used to test Learning and memory. Two doses (200 and 400 mg/kg, p.o.) of ethanolic extract of Cressa creticawere administered for 28 successive days in mice. The dose of 400 mg/kg p.o. of CCE (Cressa creticaextract) significantly enhanced Learning and memory of mice.

This dose significantly opposed the memory loss caused by Scopolamine (0.4 mg/kg, i.p.). The effect of CCE on whole Brain MDA, SOD, GSH, Catalase, NO activity was estimated to analyze how CCE shows Nootropic activity. CCE reduced wholeBrain MDA, NO levels. Antioxidant properties and presence of flavonoids in Cressa creticamay be responsible for Nootropic activity. Piracetam (200 mg/kg, i.p) was utilized as standard Nootropic agent . Hence Cressa creticaseems to be a potent candidate for Enhancing Learning and memory and it would be beneficial for the treatment of amnesia and Alzheimer’s disease.

Dangsheng Polysaccharide (radix codonopsis)

Nootropic EFFECT OF DANGSHENG, CODONOPSIS PILOSULA (FRANCH.) NANNF

Twenty Percent alcohol extract of Dangshen (DS-A) was used for facilitation of memory tested by one trail passive avoidance response (step down test) in mice and operant conditional reflex (fixed ratio) in rats. The extract reduced the impairment of acquisition of memory produced by scopolamine (SCPL), the disruption of consolidation of memory caused by sodium nitrite and the deficit of retrieval of memory induced by alcohol. And also, DS-A could improve operant conditional reflex impaired by SCPL. Similar effects were observed with the n-butyl alcohol extrect (DS-FIII), further purified from DS-A, of the agent . It suggested that the active components of Dangshen may be in DS-FIII. Effect of DS-FIII on M-cholinergic receptors were measured by radioligand receptor assay (RRA).

No specific binding; of DS-FIII to M-receptor was observed. However, 5 days after ip injection of DS-FIII higher Bmax values were observed. Effects of DS-FIII and SCPL on Ach content of cortex and hippocampus were measured by radioimmunoassay(RIA) .SCPL reduced the Ach content in cortex and hippocampus markedly. Whereas, DS-FIII could not affect the reduction and raise the Ach content.

The results suggested that one of mechanisms of action of Dangshen on memory facilitation may be related to cholinergic system. But, the mechanism of the Nootropic action of Dangshen is very complicate and need to be further studied.

Dichrocephala Integrifolia

Nootropic and neuroprotective Effects of Dichrocephala integrifolia on Scopolamine Mouse Model of Alzheimer’s Disease

Alzheimer’s disease the most common form of dementia in the elderly is a neurodegenerative disease that affects 44 millions of people worldwide. The first treatments against Alzheimer’s disease are acetylcholine sterase inhibitors; however, these medications are associated with many side effects. Dichrocephala integrifolia is a traditional herb widely used by indigenous population of Cameroon to treat and prevent Alzheimer’s disease and for memory improvement. In this study, we evaluated the effect of the decoction prepared from leaves of D. integrifolia, on scopolamine-induced memory impairment in mice. Seven groups of six animals were used. The first two groups received distilled water for the distilled water and scopolamine groups.

The four test groups received one of the four doses of the decoction of the plant (35, 87.5, 175 or 350 mg/kg p.o.) and the positive control group received tacrine (10 mg/kg), a cholinesterase inhibitor used in the treatment of Alzheimer’s disease, during 10 consecutive days. Scopolamine (1 mg/kg), a cholinergic receptor blocker, administered 30 min after treatments, was used to induce memory impairment to all groups except the distilled water group on day 10 of agent treatment. The behavioral paradigms used to evaluate the effects of the treatment were the elevated plus maze for Learning and memory, Y maze for spatial short-term memory, the novel object recognition for recognition memory and Morris water maze for the evaluation of spatial long-term memory.

After behavioral tests, animals were sacrificed and brains of a subset were used for the assessment of some biomarkers of oxidative stress (malondialdehyde and reduced glutathione levels) and for the evaluation of the acetylcholine sterase activity. From the remaining subset brains, histopathological analysis was performed. The results of this study showed that, D. integrifolia at the doses of 87.5 and 350 mg/kg significantly (p < 0.01) improved spatial short-term and long-term memory, by increasing the percentage of spontaneous alternation in the Y maze and reducing the escape latency in the Morris water maze. Furthermore, the results of histopathological evaluation showed that D. integrifolia attenuated the neuronal death in the hippocampus induced by scopolamine. The main finding of this work is that D. integrifolia improves Learning capacities and counteracts the memory impairment induced by scopolamine. Thus, D. integrifolia can be a promising plant resource for the management of Alzheimer’s disease and memory loss.

Docosahexaenoic acid (marine microalgae)

DOCOSAHEXAENOIC ACID ADMINISTRATION AMELIORATES SCOPOLAMINE-INDUCED memory IMPAIRMENT IN MICE

Eclipta Alba

Neuropharmacological profile of Eclipta alba (Linn.) Hassk

The present study deals with the investigation of standardized and phytochemically evaluated aqueous and hydroalcoholic extracts of the plant Eclipta alba for sedative, muscle-relaxant, anxiolytic, Nootropic and anti-stress activities. The hydrolyzed fraction of the aqueous extract was also subjected to similar studies in rats. The aqueous and hydroalcoholic extracts were administered in a dose of 150 and 300 mg/kg, p.o., while the hydrolyzed fraction was administered in a dose of 30 mg/kg, p.o. The findings indicated Nootropic activity of the aqueous extract (300 mg/kg, p.o.) and its hydrolyzed fraction (30 mg/kg, p.o.).

The effect of the extracts on stress -induced alterations was evaluated. The aqueous extract and the hydrolyzed fraction provided protection against cold restraint induced gastric ulcer formation and also normalized the white blood cell count in the milk induced leukocytosis challenge model. The hydroalcoholic extract on the other hand demonstrated a significant effect only in the milk induced leukocytosis challenge model. The results point towards the potential neuropharmacological activity of the plant Eclipta alba as a Nootropic and also having the property of attenuating stress induced alterations. Further neurochemical investigations can unravel the mechanism of action of the plant agent with respect to Nootropic activity and help to establish the plant in the armamentarium of Nootropic agents.

Eclipta Prostrata

The butanol fraction of Eclipta prostrata (Linn) increases the formation of Brain acetylcholine and decreases oxidative stress in the Brain and serum of cesarean-derived rats

Eclipta prostrata has been used as a traditional medicinal plant to prevent dementia and to enhance memory in Asia. Its potential as a Nootropic and as an Antioxidant have been reported in mice. We hypothesized that Eclipta may affect the formation of neurotransmitter s and the inhibition of oxidative stress. Charles River cesarean-derived rats (male, 180 ± 10 g) were fed experimental diets supplemented with 0 mg (control), 25 mg (E25), 50 mg (E50), or 100 mg (E100) of a freeze-dried butanol fraction of E prostrata per kilogram of diet for 6 weeks. The acetylcholine level was significantly increased by 9.6% and 12.1% in the brains of E50 and E100 groups, respectively, as compared with the control group that was fed standard diet alone.

The acetylcholine esterase activity was significantly increased by 13.1% and 19.7% in the brains of E50 and E100 groups, respectively, compared with the control group. Monoamine oxidase-B activity was significantly decreased by 10.5% in the brains of the E100 group, and the superoxide radical level was significantly reduced by 9.4% in the serum of the E100 group compared with the control group. Superoxide dismutase activity was significantly increased by 9.6% and 11.6% in the serum of E50 and E100 groups, respectively, compared with the control group. These results clearly demonstrate the effects of E prostrata on the formation of acetylcholine in the Brain and the inhibition of oxidative stress in the Brain and serum of rats. These findings may have implications for preventing dementia and Enhancing memory function in humans.

Embelin

Amelioration of Cognitive Deficit by Embelin in a Scopolamine-Induced Alzheimer’s Disease-Like Condition in a Rat Model

Embelin (2,5-dihydroxy-3-undecyl-1,4-benzoquinone) is one of the active components (2.3%) found in Embelia ribes Burm fruits. As determined via in vitro AChE inhibition assay, embelin can inhibit the acetylcholine sterase enzyme. Therefore, embelin can be utilized as a therapeutic compound, after further screening has been conducted for its use in the treatment of Alzheimer’s disease (AD). In this study, the Nootropic and anti-amnesic effects of embelin on scopolamine-induced amnesia in rats were evaluated. Rats were treated once daily with embelin (0.3 mg/kg, 0.6 mg/kg, 1.2 mg/kg) and donepezil (1 mg/kg) intraperitoneally (i.p.) for 17 days. During the final 9 days of treatment, a daily injection of scopolamine (1 mg/kg) was administered to induce Cognitive deficits. Besides that, behavioral analysis was carried out to assess the rats’ Learning and memory functions.

Meanwhile, hippocampal tissues were extracted for gene expression, neurotransmitter, and immunocytochemistry studies. Embelin was found to significantly improve the recognition index and memory retention in the novel object recognition (NOR) and elevated plus maze (EPM) tests, respectively. Furthermore, embelin at certain doses (0.3 mg/kg, 0.6 mg/kg, and 1.2 mg/kg) significantly exhibited a memory–Enhancing effect in the absence of scopolamine, besides improving the recognition index when challenged with chronic scopolamine treatment. Moreover, in the EPM test, embelin treated rats (0.6 mg/kg) showed an increase in inflection ratio in Nootropic activity.

However, the increase was not significant in chronic scopolamine model. In addition, embelin contributed toward the elevated expression of BDNF, CREB1, and scavengers enzymes (SOD1 and CAT) mRNA levels. Next, pretreatment of rats with embelin mitigated scopolamine-induced neurochemical and histological changes in a manner comparable to donepezil. These research findings suggest that embelin is a Nootropic compound, which also possesses an anti-amnesic ability that is displayed against scopolamine-induced memory impairment in rats. Hence, embelin could be a promising compound to treat AD.

Epicatechin

Evaluation of Nootropic and Anti-Nociceptive Activity of Green Tea inComparison with Medhya Rasayana

Etiracetam

memory retrieval deficits: Alleviation by etiracetam, a Nootropic agent

Etiracetam, a nonanaleptic agent related to the Nootropic substance piracetam, was found to facilitate memory retrieval in rats in several experimental situations, when injected 30 min prior to retention testing. The agent was active when memory deficits were induced by electroconvulsive shock, undertraining, or by a long training-to-test interval. The behavioral paradigms included a one-trial inhibitory avoidance task and a complex multitrial, spatially discriminated approach task. The clinical interest of agents which facilitate retrieval processes is also discussed.

Evolvulus Alsinoides

COMPARATIVE Nootropic EFFECT OF EVOLVULUS ALSINOIDES AND CONVOLVULUS PLURICAULIS.

The aim of the present study was to highlight the comparative Nootropic effects of Evolvulus alsinoides and Convolvulus pluricaulis using two validated models of memory namely jumping box and elevated plus maze. Evolvulus alsinoides and Convolvulus pluricaulis are regarded as the botanical source of Shankhpushpi along with Clitorea ternatea and Canscoradecussata. Shankhpushpi, an important agent of indigenous system of medicine is known as a Brain tonic, alterative and laxative.

However various authors on Indian medicinal plants have different opinion about its correct botanical source. Rats were treated orally with vehicle (2% Tween 80 suspension), standard treatment (Piracetam, 200mg/kg body weight), alcoholic extracts of Evolvulous alsinoides and Convolvulus pluricaulis (250mg/kg body weight) respectively, one hour prior to the evaluation of behavioral parameters. The results indicate that alcoholic extracts of Evolvulous alsinoidesexhibited superior Nootropic activity as compared to Convolvulus pluricaulis in terms of time spent in the enclosed arm in plus maze model and the mean avoidance response on the jumping box model.

Fasoracetam

Fasoracetam – An anxiety -Reducing Nootropic

Fasoracetam is a fascinating Nootropic with a novel mechanism of action (relative to the other racetams). It has powerful anti-anxiety and anti-depressant effects, in addition to its ability to improve memory and focus.

As more and more research is done on fasoracetam, the more likely it will continue to grow in popularity. Right now, it’s hard to find and a little more expensive than most of the other racetams.

Ficus Religiosa

A TRADITIONAL APPROACH TO HERBAL Nootropic AGENTS: ANOVERVIEW

Nootropic agents used as a memory enhancer can improve thinking, memory, and alertness in people with Alzheimer’s disease andother disease that affect the mind. Memory is perhaps the most vital of all aspects that differentiates human beings from other animals. However, memory can become faulty due to several reasons, and in that case the person is not able to make full use of his or her potentials. Since ages, agents and natural remedies have been prescribed to enhance memories in people. 4 million people are thought to be suffering from age related memory and increased risk of developing Alzheimer’s disease.

Although several Nootropic agents are available to treat memory problems. In recent years research on medicinal plants have been studied for Nootropic activity. Bacopa monnieri (Brahmi), Evolvulus alsinoides (Shankhpushpi),Withania somnifera (Ashwagandha),Acorus calamus(Bach)etc.,are used as a memory enhancer agents. The abstract refers to several plants with their activity. The main aim of this article isto give up the data reviews on plants withNootropic properties.

Filipendula Vulgaris

Nootropic Effect of Meadowsweet (Filipendula vulgaris) Extracts

The effects of the extracts of the aboveground parts of Filipendula vulgaris Moench on the Behavior and memory of mice after hypoxic injury and their physical performance in the open-field test were studied using the models of hypoxia in a sealed volume, conditioned passive avoidance response (CPAR), and forced swimming with a load. The extracts improved animal resistance to hypoxia, normalized orientation and exploration activities, promoted CPAR retention after hypoxic injury, and increased physical performance. Aqueous extract of meadowsweet had the most pronounced effect that corresponded to the effect of the reference agent piracetam. These effects were probably caused by modulation of hippocampal activity.

Foeniculum Vulgare

cholinergic Basis of memory -Strengthening Effect of Foeniculum vulgare Linn.

alzheimer’s disease is a neurodegenerative disorder associated with a decline in Cognitive abilities. dementia is one of the age-related Mental problems and a characteristic symptom of alzheimer’s disease. Nootropic agents are used in situations where there is organic disorder in Learning abilities. The present work was undertaken to assess the potential of Foeniculum vulgare Linn. extract as a Nootropic and anticholinesterase agent in mice. Methanolic extract of the whole plant of F. vulgare Linn. administered for eight successive days ameliorated the amnesic effect of scopolamine (0.4 mg/kg) and aging- induced memory deficits in mice. The passive avoidance paradigm served as the exteroceptive behavioral model for assessing memory. F. vulgare extract increased step-down latency and acetylcholine sterase inhibition in mice significantly. Hence, F. vulgare can be employed in treatment of Cognitive disorders such as dementia and alzheimer’s disease.

Galantamine

Evaluation of Nootropic effects of galantamine and sildenafil as a combination in mice

Cognitive decline is one of the age related Mental problems and a characteristic symptom of various neurodegenerative disorders. Nootropic effects of combination of sildenafil and galantamine was evaluated in different Learning and memory paradigms viz. Elevated plus maze (EPM) and Morris water maze (MWM) against scopolamine induced Cognitive impairment. Moreover, the influence on central cholinergic activity via estimating the whole Brain acetylcholine sterase enzyme was also assessed. Sildenafil (8 mg/kg, i.p.) and galantamine (3 mg/kg, i.p.) were administered per se to Swiss albino mice for successive 14 days. In addition, combination of sildenafil (4 mg/kg, i.p.) and galantamine (1.5 mg/kg, i.p.) were administered. Scopolamine (1 mg/kg, i.p.) was used to induce amnesia. Inflexion ratio and time spent in target quadrant were determined in EPM and MWM, respectively.

Further, whole Brain acetylcholine sterase enzyme was estimated through Ellman’s method. Treatment with sildenafil and galantamine combination significantly increased inflexion ratio and time spent in target quadrant in EPM and MWM, respectively. Combination treatment also showed reduction in Brain acetylcholine sterase enzyme activity when compared separately against sildenafil and galantamine per se. The present study results suggest the augmentation of benefits of galantamine and sildenafil combination in the treatment of Cognitive impairments.

Gamma-Aminobutyric Acid (Gaba) 56-12-2

Nootropic properties of gamma-aminobutyric acid derivatives

Experiments on rats and mice were made to study the antianamnestic and antihypoxic effects of some GABA derivatives. Cetyl GABA, sodium and lithium hydroxybutyrates and phenibut were shown to be able to decrease the retrograde amnesia caused by electroshock in passive avoidance performance. As regards the degree of the antianamnestic effect, the above-mentioned non-cyclic derivatives of GABA are not inferior to the standard Nootropic agent piracetam, a derivative of cyclic GABA. antihypoxic activity of sodium hydroxybutyrate, cetyl GABA, phenibut and lioresal studied in experimental hypoxic hypoxia compares very favourably with that of piracetam.

The compounds under consideration manifest their protective action against damaging factors in doses which do not provoke muscle relaxation or any types of central depression. According to the data obtained one may conclude that the Nootropic effect is exhibited by not only piracetam, a derivative of cyclic GABA, but also by some of its non-cyclic derivatives.

Ginkgo Biloba extract

The acute Nootropic effects of Ginkgo biloba in healthy older human subjects: a preliminary investigation

Ginkgo biloba has been shown to have chronic memory Enhancing effects in healthy subjects and patients with dementia. There is limited research on the acute Nootropic effects of Ginkgo biloba in humans. The current study aimed to examine the acute effects of Ginkgo biloba (120 mg) on memory functioning in healthy older volunteers using the Cognitive agent research (CDR) battery of memory tests and the Rey auditory verbal Learning task (AVLT).

The study was a double‐blind placebo‐controlled design, with each participant tested under both placebo and Ginkgo biloba treatment conditions. Testing was conducted pre‐ and 90 min post‐agent administration for each treatment condition. Treatment conditions were separated by a 7 day wash out period. No acute effects of Ginkgo biloba were found for any of the memory tests examined. The findings suggest that 120 mg of Ginkgo biloba has no acute Nootropic effects in healthy older humans.

Hibiscus Sabdariffa

Nootropic Activity of Calyces of Hibiscus Sabdariffa Linn

Nootropic acitivity of calyces of Hibiscus sabdariffa Linn. was studied in mice. Elevated plus maze and passive avoidance paradigm were employed to evaluate Learning and memory parameters. Scopolamine (0.4 mg/kg, i.p.) was used to induce amnesia in mice. The aqueous extracts of calyces of Hibiscus sabdariffa (HS-100 and 200 mg/kg, p.o.) significantly attenuated amnestic deficits induced by scopolamine (0.4 mg/kg, i.p.) and natural aging. HS (100 and 200 mg/kg) decreased the transfer latencies and increased step down latencies significantly in the aged mice and scopolamine induced amnesic mice as compared with Piracetam (200 mg/kg, i.p.). To delineate the possible mechanism through which H. sabdariffa elicits the anti-amnesic effects, we studied its influence on central cholinergic activity by estimating the whole Brain acetylcholine sterase activity. H. sabdariffa significantly decreased acetyl cholinesterase activity in mice.

The results indicate that, the aqueous extract of calyces of H. sabdariffa might prove to be a useful memory restorative agent in the treatment of dementia seen in elderly. The underlying mechanism of action can be attributed to its anti acetylcholine sterase property.

Hovenia Dulcis

The Nootropic components of Hovenia dulcis.

Objective: To investigate the Nootropic components of Hovenia dulcis.

Methods: Six saponins were isolated from Hovenia dulcis and Step-down test was used to examine the memory ability of mice. The escape latency and the times of wrong performance within 3 min were used to evaluate the memory ability of mice. To study the effects of saponins on learning and memory in mice, we divided the mice into 6 groups; youth group, aged group, aged plus piracetam (0.3 g/kg) group, aged plus saponins (0.6 g/kg) group, aged plus saponins (0.3 g/kg) group, and aged plus saponins (0.15 g/kg) group. To study the influence of saponins on impairment of memory acquirement, consolidation, and reoccurrence (induced by scopolamine, sodium nitrite and 40% ethanol, respectively), mice were also divided into the following 7 groups: control group, untreated group, piracetam group (0.3 g/kg), compound 3 group (0.3 g/kg), compound 4 group (0.3 g/kg), compound 5 group (0.3 g/kg), and compound 6 group (0.3 g/kg).

Results: The chemical structures of six saponins were elucidated as 3-O-stigmasterol-(6-O-palmitoyl)-β-D-glucopyranoside (1), β-daucosterin (2), hovenidulcioside A₁ (3), hoduloside I (4), hoduloside IV (5), and saponins C₂ (6). Among them, compounds 1 and 2 were isolated from this plant for the first time. Compounds 5 and 6 had Enhancing effect on the Learning and memory ability of natural senile mice, and they could improve the impairment of memory acquirement, consolidation and recurrence in mice induced by scopolamine, sodium nitrite and 40% ethanol, respectively.

Conclusion: The aglycone of jujubogenin might be the main saponins contributing to the Nootropic effect of total saponins from Hovenia dulcis.

Huperzine A (Lycopodium serratum Thunb)

Huperzine A, a Nootropic alkaloid, inhibits N-methyl-D-aspartate-induced current in rat dissociated hippocampal neurons

Hypericin (Hypericum Perforatum)

Hypericum perforatum as a Nootropic agent : enhancement of retrieval memory of a passive avoidance conditioning paradigm in mice

Depression, among other non-Cognitive symptoms, is common in patients with dementia. The effect of Hypericum perforatum (St. John’s Wort) extract, with well-documented antidepressant activity, was tested on memory retrieval 24 h after training on a one-trial passive avoidance task in mice. Acute administration of Hypericum extract (4.0, 8.0, 12.0, and 25.0 mg/kg i.p.) before retrieval testing increased the step-down latency during the test session. The same doses of Hypericum extract, on the other hand, failed to reverse scopolamine-induced amnesia of a two-trial passive avoidance task. The involvement of serotonergic, adrenergic, and dopamine rgic mechanisms in the facilitatory effect of Hypericum extract on retrieval memory was investigated.

Pretreatment of the animals with serotonergic 5-HT1A receptor antagonist (−)-pindolol (0.3, 1.0, and 3.0 mg/kg), serotonergic 5-HT2A receptor blocker spiperone (0.01, 0.03, and 0.1 mg/kg), alpha adrenoceptor antagonist phentolamine (1, 5, and 10 mg/kg), beta receptor antagonist propranolol (5, 7.5, and 10 mg/kg), dopamine rgic D1 receptor antagonist SCH 23390 (0.01, 0.05, and 0.1 mg/kg), and dopamine rgic D2 receptor antagonist sulpiride (5, 7.5, and 10 mg/kg) revealed the involvement of adrenergic and serotonergic 5-HT1A receptors in the facilitatory effect of Hypericum extract on retrieval memory.

It is concluded that Hypericum extract may be a better alternative for treatment of depression commonly associated with dementia than other antidepressants known to have anticholinergic side effects causing delirium, sedation and even exacerbating already existing impaired cognition. In dementias of old age, Hypericum perforatum would, therefore, serve as one medication targeting both depression and amnesia with lower potential side effects.

Idebenone

Effect of idebenone and various Nootropic agents on lipid peroxidation in rat Brain homogenate in the presence of succinate

The effects of idebenone and various Nootropic agents on lipid peroxidation in rat Brain homogenate were examined. Idebenone inhibited lipoperoxide (LPO) production in Brain homogenate in a concentration -dependent manner, with an IC50 of 38 microM. The inhibition was strongly enhanced (about 100-fold) by adding succinate, a substrate in the mitochondrial respiration. The optimal concentration of succinate was 0.5 mM. Inhibition of lipid peroxidation in Brain homogenate by various Nootropic agents in the presence or absence of succinate was then examined. agents added to the Brain homogenate at 100 microM in the absence of succinate inhibited LPO production in the order: idebenone greater than vinpocetine greater than bifemelane greater than indeloxazine greater than calcium hopantenate. However, when the agents were added at 1 microM in the presence of succinate, only idebenone demonstrated inhibition.

These results suggest that although almost all of the agents tested inhibit lipid peroxidation in Brain homogenate, only idebenone is activated by succinate, the other agents being insensitive to this compound.

Ipomoea Aquatic Forsk

Nootropic EFFECT OFIPOMOEA AQUATICAFORSK INRAT HIPPOCAMPUS

Alzheimer’s disease (AD) is a primary degenerative disease of the central nervous system.The progression of disease will ultimately lead to dementia, behavioral and Cognitive impairments.AD ischaracterized by selective neuron al cell death, the presence of extra cellular amyloid deposits in the coreof neuritic plaques and the formation of intraneuron al neurofibrillary tangles in the Brain of affectedindividuals.AD affects up to 15% of people over age 65 years and nearly half of people age 85 years.Prevalence rates for more than 20 million people worldwide including 4.5 million Americans.In AD, thesevere loss of cholinergic neurons in the nucleus basalis and associated areas that form the cholinergicforeBrain area and their projections to the cerebral cortices are marked with decreased levels ofacetylcholine (ACh). The aim of the study is to elvaluate the acetylcholine Enhancing activity ofmethanol leaf extract ofIpomoea aquaticaForsk(MEIA), is known to posses various therapeuticproperties.We found that treatment with 200 and 400 mg/kg of MEIA, for 30 days in neonatal and youngadult age groups of rat, significantly increased acetylcholine (ACh) content in their hippocampus ascompared to age matched controls. Increase in ACh content in their hippocampus may be theneurochemical basis for their improved learning and memory.

Juniperus Recurva

Exploring the Nootropic effect of Juniperus recurva extract: Possible involvement of acetylcholine sterase inhibition

Background: Currently, available therapy for the treatment of memory impairment is far from satisfactory. Therefore, the agents of natural origin may serve as potential therapies. Objective: The present study was designed to evaluate the memory –Enhancing effect of Juniperus recurva extract.

Materials and Methods: The methanol extract of J. recurva was prepared by Soxhlet extraction and characterized by high-performance liquid chromatography (HPLC). The in vitro Antioxidant activity of the extract was corroborated by diphenyl picryl hydrazine scavenging, nitric oxide scavenging, metal chelating, and reducing power activity.  Memory impairment was induced by the administration of scopolamine (1 mg/kg i.p) on 3 consecutive days to mice and assessment of memory acquisition and memory retention was done using Morris water maze test, passive avoidance test, elevated plus maze test, and light and dark box test, motor coordination was evaluated using the rotarod test and inclined plane test; and depression was evaluated by forced swim test. Serum acetylcholine sterase (AChE) activity was quantified by Ellman’s method.

Results: The HPLC analysis of J. recurva extract revealed gallic acid as a prominent peak. The extract was found to have an excellent antioxidant effect in all the tests employed. The in vivo studies revealed memory Enhancing and improve d motor coordination activity of the extract in mice. Serum AChE activity was decreased on the administration of the extract.

Conclusion: The inhibition of the AChE enzyme contributes to the memory–Enhancing activity of J. recurva extract.

Leontopodium Alpinum

Extracts and constituents of Leontopodium alpinum enhance cholinergic transmission: Brain ACh increasing and memory improving properties

Leontopodium alpinum (‘Edelweiss’) was phytochemically investigated for constituents that might enhance cholinergic neurotransmission. The potency to increase synaptic availability of acetylcholine (ACh) in rat Brain served as key property for the bioguided isolation of cholinergically active compounds using different chromatographic techniques. The dichlormethane (DCM) extract of the root, fractions and isolated constituents were injected i.c.v. and the effect on Brain ACh was detected via the push–pull technique. The DCM extract enhanced extracellular ACh concentration in rat Brain and inhibited acetylcholine sterase (AChE) in vitro. The extracellular level of Brain ACh was significantly increased by the isolated sesquiterpenes, isocomene and 14-acetoxyisocomene, while silphiperfolene acetate and silphinene caused a small increasing tendency. Only silphiperfolene acetate showed in vitro AChE inhibitory activity, thus suggesting the other sesquiterpenes to stimulate cholinergic transmission by an alternative mechanism of action. Isocomene was further investigated with behavioural tasks in mice. It restored object recognition in scopolamine-impaired mice and showed Nootropic effects in the T-maze alternation task in normal and scopolamine-treated mice.

Additionally, this sesquiterpene reduced locomotor activity of untreated mice in the open field task, while the activity induced by scopolamine was abolished. The enhancement of synaptic availability of ACh, the promotion of alternation, and the amelioration of scopolamine-induced deficit are in accordance with a substance that amplifies cholinergic transmission. Whether the mechanism of action is inhibition of AChE or another pro-cholinergic property remains to be elucidated. Taken together, isocomene and related constituents of L. alpinum deserve further interest as potential antidementia agents in Brain diseases associated with cholinergic deficits.

Leuzea Carthamoides

A TRADITIONAL APPROACH TO HERBAL Nootropic AGENTS: ANOVERVIEW

Nootropic agents used as a memory enhancer can improve thinking, memory, and alertness in people with Alzheimer’s disease andother disease that affect the mind. memory is perhaps the most vital of all aspects that differentiates human beings from other animals. However, memory can become faulty due to several reasons, and in that case the person is not able to make full use of his or her potentials. Since ages, agents and natural remedies have been prescribed to enhance memories in people. 4 million people are thought to be suffering from age related memory and increased risk of developing Alzheimer’s disease.

Although several Nootropic agents are available to treat memory problems. In recent years research on medicinal plants have been studied for Nootropic activity. Bacopa monnieri (Brahmi), Evolvulus alsinoides (Shankhpushpi),Withania somnifera (Ashwagandha),Acorus calamus(Bach)etc.,are used as a memory enhancer agents. The abstract refers to several plants with their activity. The main aim of this article isto give up the data reviews on plants withNootropic properties.

Marsilea Minuta

Effect of standardized extract of Marsilea minuta on Learning and memory performance in rat amnesic models

Meclofenoxate

Changes in Brain biogenic monoamines induced by the Nootropic agents adafenoxate and meclofenoxate and by citicholine (experiments on rats)

1. The effects of Adafenoxate (Adf), meclofenoxate (Mf) and citicholine (CCh) administered at a daily dose of 100 mg/kg for 7 days on the levels of noradrenaline (NA), dopamine (DA) and serotonin (5-HT) in the frontal cerebral cortex, striatum, hippocampus and hypothalamus of rats were studied.

2. Adafenoxate increased the NA level in the striatum and decreased it in the hypothalamus; it increased the DA level in the cerebral cortex and hypothalamus and decreased it in the striatum; it increased the 5-HT level in the cerebral cortex and decreased it in the hippocampus.

3. Meclofenoxate decreased the NA level in the cerebral cortex and hypothalamus; it increased the DA level in the hippocampus and hypothalamus and the 5-HT level in the cerebral cortex, striatum, hippocampus and hypothalamus.

4. Citicholine increased the NA level in the cerebral cortex and hypothalamus; it increased the DA level in the striatum and the 5-HT level in the cerebral cortex, striatum and hippocampus.

5. An attempt is made to explain some similarities and differences in the behavioral effects of the agents tested (and those observed in other studies) by the changes they induce in Brain biogenic monoamines.

Mimosa Pudica

Adaptogenic and Nootropic activity of Mimosa pudica in albino wistar rats

Aim: To evaluate the adoptogenic activity of ethanolic extract of Mimosa pudica L. in chronic alzheimer’s model.

Materials and Methods: The dried whole part of the plant were subjected to hot continuous extraction method using ethanol as solvent and were standardized using pharmacognostical and phytochemical screening. The in-vitro Antioxidant studies were conducted by super oxide scavenging assay and hydrogen peroxide scavenging assay. Satisfactory IC ₅₀ values were obtained for both assays. Dose selection for in-vivo study was made on the basis of already conducted acute toxicity studies (500 mg/kg body weight). Oral administration of extract of M. pudica was continued for 21 consecutive days. Adaptogenic activity was assessed by using oral dose of 500 mg/kg of EEMP and 2 mg/kg diazepam as test and standard compound respectively.

Results: There was a significant improve ment in memory, which was observed from the test paradigms viz., morris water maze, radial arm maze. Forced swim test was used as the observation paradigm for the adaptogenic activity and EEMP caused significant reduction in swimming endurance time.

Conclusion: It was concluded that ethanolic extract of M. pudica at the dose of 500 mg/kg p.o produces potential changes in chronic alzheimer’s model and stress.

Moringa Oleifera Leaves

Nootropic activity of Moringa oleifera leaves

Objective: To study the Nootropic activity of leaves of Moringa oleifera.

Methods: Toluene–ethylacetatefraction of methanolic extract of Moringa oleifera (MOE) leaves was assessed for its Nootropic activityusing passive shock avoidance paradigm and elevated plus maze. MOE ( 50 and 100 mg/kg ) was comparedwith Piracetam (100 mg/kg ). Scopolamine (1 mg/kg) was used to induce Cognitive dysfunction. Theextract was also studied for its effect on gross behaviour.

Results: MOE (50 and 100 mg/kg ) significantlydecreased Transfer Latency(TL) on Day 2. The extract reduced the latency to reach the SFZ and thenumber of mistakes. No adverse effects were observed upto a dose of 200 mg/kg.

Conclusion: Thus theleaves of Moringa oleifera possess a potential for exploring the Nootropic principle.

Murraya Koenigii

Nootropic Potential of Murraya koenigii leaves in Rats

Murraya koenigii leavescommonly known as ‘curry patta’ are routinely added to Indian gravy andvegetarian dishes by south Indian as a favourate condiment. The present study was undertaken toinvestigate the effects of Murraya koenigii leaves (MKL) on memory in rats. Elevated plus-maze andHebb-Williams maze served as the exteroceptive behavioral models for testing memory. Diazepam-,scopolamine- and ageing-induced amnesia served as the interoceptive behavioral models. MKL fedorally to various groups of young and aged rats with diet containing 2, 4 and 8% w/w of MKL for 30days consecutively were investigated. The MKL diets produced a significant dose-dependentimprove ment in memory scores of young and aged rats and significantly reduced the amnesiainduced by scopolamine (0.4 mg/kg, i.p.) and diazepam (1 mg/kg, i.p.). Therefore, it would be worthwhile to specifically investigate the therapeutic potential of MKL in the management of dementia patients.

Nardostachys Jatamansi

Nardostachys jatamansi improves Learning and memory in Mice

Cure of Cognitive disorders such as amnesia, attention deficit, and alzheimer’s disease is still far from being realized in the field of medicine. Nootropic agents such as piracetam, aniracetam, and choline esterase inhibitors like donepezil are being used for improving memory, mood, and behavior, but the resulting side effects associated with these agents have made their applicability limited. In Ayurveda, the roots of Nardostachys jatamansi have been clinically employed for their anti-ischemic, Antioxidant, anticonvulsant, and neuroprotective activities. The present study was undertaken to assess the potential of N. jatmansi as a memory enhancer. The elevated plus maze and the passive avoidance paradigm were employed to evaluate learning and memory parameters. Three doses (50, 100, and 200 mg/kg, p.o.) of an ethanolic extract of N. jatamansi were administered for 8 successive days to both young and aged mice. The 200 mg/kg dose of N. jatmansi ethanolic extract significantly improved learning and memory in young mice and also reversed the amnesia induced by diazepam (1 mg/kg, i.p.) and scopolamine (0.4 mg/kg, i.p.).

Furthermore, it also reversed aging-induced amnesia due to natural aging of mice. As scopolamine-induced amnesia was reversed, it is possible that the memory improve ment may be because of facilitation of cholinergic transmission in the brain. Hence, N. jatmansi might prove to be a useful memory restorative agent in the treatment of dementia seen in elderly persons. The underlying mechanism of action can be attributed to its Antioxidant property.

Nefiracetam

EFFECTS OF THE Nootropic NEFIRACETAM ON RAT hippocampal GABA RELEASE

The ventral hippocampus plays an important role in generalizing seizures. We have previously reported antiepileptic effects of the Nootropic nefiracetam in epileptic mutant EL mice with an ED₅₀ of 7.5 mg/kg in p.o. In the present study, we used the microdialysis method for exploring the role of hippocampal GABAergic system under the influence of nefiracetam. Application of nefiracetam (10 mg/kg, p.o.) markedly increased GABA release to a level 3 times as high as the base line level. Then, the release gradually decreased to almost zero-level in 2 hr. With a higher dose (30 mg/kg, p.o.) of nefiracetam, GABA release sharply decreased to a quarter of the base line level and then gradually recovered in 40 min. An after-potentiation (about 1.5x base line level) was observed for 20 min.

The results suggest that nefiracetam at low, clinical doses potentiates hippocampal GABA release whereas at high doses it suppresses the GABA release.

Nigella Sativa

Black seed oil ameliorated scopolamine-induced memory dysfunction and cortico-hippocampal neural alterations in male Wistar rats

Spontaneous alteration Behavior (SAB) is a viable measure of memory .37 BSO from this study significantly increased percentage alternation when given only and/or after scopolamine, thus confirming its anti-amnesic efficacy and its Nootropic property. This effect is strengthened by the report of Rashid and colleagues where a similar increase in percentage alternation was reported of thymoquinone, the active component of BSO against scopolamine38 also its Enhancing effect on spatial working memory performance on the radial arm maze.

Noopept

The Nootropic and neuroprotective proline-containing dipeptide noopept restores spatial memory and increases immunoreactivity to amyloid in an alzheimer’s disease model

The effects of the novel proline-containing Nootropic and neuroprotective dipeptide, noopept (GVS-111, N-phenylacetyl-L-prolylglycine ethyl ester) were investigated in NMRI mice following olfactory bulbectomy. We have shown previously that these animals developed alzheimer’s disease (AD)-like behaviour, morphology and biochemistry including impairment of spatial memory , regional neuron al degeneration and elevated Aβ peptide Brain levels. In the current investigation, spatial memory was assessed using the Morris water maze and serum antibodies to in vitro morphologically characterized amyloid structures of both Aβ_(25—35) peptide and equine lysozyme, as well as to neurotrophic glial factor S100b, were analyzed by enzyme-linked immunosorbent assay (ELISA). Noopept (administered at a dose of 0.01 mg/kg for a period of 21 days and during a further 5 days training) restored spatial memory and increased serum antibody levels to oligomers of Aβ_(25—35) peptide but not to equine lysozyme amyloid or S100b protein in bulbectomized animals. The positive immunotropic effect of noopept to Aβ_(25—35) peptide prefibrillar aggregates was more marked in sham-operated compared to the bulbectomized subjects which were characterized by an overall suppression of immunoreactivity. Enhancement of the immune response to Aβ_(25—35) peptide prefibrils caused by noopept may attenuate the neurotoxic consequences of amyloid fibrillization and also be associated with an improve ment in spatial memory in bulbectomized mice. These actions of noopept, combined with it’s previously reported neuroprotective and cholinomimetic properties, suggests that this dipeptide may well be useful for improving Cognitive deficits induced by neurodegenerative diseases.

Nyctanthes

Anti-stress anxiolytic and Nootropic activity of Nyctanthes arbour tritis leaves

Reports suggest that stress is the most common etiological factor in CNS disorders like anxiety, Schizophrenia, Parkinson’s disease and Alzheimer disease for which effective treatment strategies are inadequate due to complexities of the ailment and the limitations of allopathic medications. There are scanty reports¹ on the putative Neuro pharmacological effects of the leaves of Nyctanthes arbor tristis Linn. (Family: Oleaceae) [NAT]; hence the present work investigated gamut of its neuro-pharmacological effects. The methanolic extract was evaluated for anxiolytic activity using plus maze model, open field test and light dark model. Further, the Nootropic potential² of extract was evaluated using Morris water maze test and plus maze model. Antistress potential³ was evaluated in Wistar rats by subjecting the animals to chronic cold restraint stress followed by biochemical estimation of plasma corticosterone, glucose, triglycerides; dopamine, 5-Hydroxy Tryptamine and nor epinephrine from brain. Diazepam 1mg/kg was used as a positive control. One-way ANOVA followed by Dunnett’s test was applied for statistical significance.

Pretreatment with NAT extract resulted in preference to open arm in plus maze test, increased exploratory Behavior in open field test and increased number of crossings in light dark model. Further it improved Cognitive function with respect to spatial and working memory processes. The treatment with NAT extract ameliorated the stress -induced variations in the biochemical levels of corticosterone, glucose, triglycerides; dopamine , 5-HT and nor epinephrine. In conclusion, the NAT extract exhibited anxiolytic, antistress and Nootropic activity with utility in oxidative Cognitive impairment due to its Antioxidant potential.

Ocimum Sanctum leaf

Evaluation of Nootropic potential of Ocimum sanctum Linn. in mice

dementia is one of the age related Mental problems and a characteristic symptom of various neurodegenerative disorders including Alzheimer’s disease. Certain agents like diazepam, barbiturates and alcohol disrupt Learning and memory in animals and man. However, a new class of agents known as Nootropic agents is now used in situations where there is organic disorder in Learning abilities. The present work was undertaken to assess the potential of O. sanctum extract as a Nootropic and anti-amnesic agent in mice. Aqueous extract of dried whole plant of O. sanctum ameliorated the amnesic effect of scopolamine (0.4 mg/kg), diazepam (1 mg/kg) and aging induced memory deficits in mice. Elevated plus maze and passive avoidance paradigm served as the exteroceptive behavioral models. O. sanctum extract decreased transfer latency and increased step down latency, when compared to control (piracetam treated), scopolamine and aged groups of mice significantly. O. sanctum preparations could of beneficial in the treatment of Cognitive disorders such as dementia and Alzheimer’s disease.

Gallic acid (Olive Olea europaea L. Fruit)

Nootropic and Anti-anxiety Effects of Olive Oil: Relationship with dopamine and Serotonin Metabolism

In recent years, interest in the use of nutraceuticals hasrisen substantially. Olive oil has been shown to produce anumber of therapeutically important effects due to itsAntioxidant property. The present study concernsneurochemical and behavioral effects of long termadministration of low and high doses (0.1 mL/kg and 0.25mL/kg) of olive oil and associated Antioxidant effects inrats. Long term administration of low dose of olive oilincreased motor activity in an open field, decreasedanxiety in elevated plus maze test, and enhanced memory in Morris water maze test.

Whole Brain levels of serotoninincreased with low dose of olive oil while homovanillicacid (HVA), a metabolite of dopamine increased with bothdoses of olive oil. Low dose of olive oil increased glutathione peroxidase activity whereas high dose of oliveoil decreased malondialdehyde levels in plasma. Theresults show that particularly low doses of olive oil reduceanxiety and improve Learning and memory together withAntioxidant properties, Brain dopamine and serotoninalso play important role in the therapeutically importanteffects of olive oil.

Oxiracetam

Brain Entry and Direct Central Pharmacological Effects of the Nootropic agent Oxiracetam

Oxiracetam administered systemically to rats as a ¹⁴C radiolabelled agent (200 mg/kg p. o. or 100 mg/kg intra-artery) enters the Brain and is found unmetabolized above all in some of the Brain areas functionally involved in the modulation of Cognitive processes. Among the Brain areas examined, the largest amount of compound was recovered in septum, followed by hippocampus; a smaller amount was found in cerebral cortex and striatum. ¹⁴C oxiracetam administered directly into the lateral ventricles of the Brain presented a similar pattern of distribution, indicating that the tropism of the agent for the above Brain areas does not depend on its route of administration. The amounts of oxiracetam that could reach the Brain after systemically administered pharmacologically active doses were also estimated.

These amounts (1.9 to 19 nmols/rat) were delivered through a permanently implanted cannula, into the lateral ventricles of the brains of conscious, freely moving rats. In this experimental condition oxiracetam dose-dependently antagonized the amnesia induced by scopolamine (0.66 mg/kg s. c.). The above findings clearly indicate that oxiracetam enters the Brain and directly exerts its pharmacological activity there.

Panax Ginseng Ginsenoside

Study on the Nootropic mechanism of ginsenoside Rb1 and Rg1–influence on mouse Brain development

Weaning mice were supplied drinking water containing Rb1 or Rg1 0.125 or 0.25 mg.ml-1 for 4 weeks. Rb1 (28.6 and 56.1 mg.kg-1) and Rg1 (27.4 and 53.9 mg.kg-1) were found to accelerate young mice body and Brain development as well as facilitate memory acquisition in step down and step through avoidance response tests. With the technique of quantitative measurement synapses, we found for the first time that Rb1 and Rg1 administration for 4 weeks can increase synapse number in the hippocampal CA3 region of mice. This is the morphological basis for explaining Rb1 and Rg1 induced facilitation of Learning and memory.

Passiflora Actinia

Possible involvement of GABA_A-benzodiazepine receptor in the anxiolytic-like effect induced by Passiflora actinia extracts in mice

Hydroethanol (HE) and methanol (ME) extracts obtained from the leaves of Passiflora actinia Hooker were evaluated for behavioral effects in mice. Single-dose oral administration of HE (300 and 600 mg/kg) or ME (100 and 300 mg/kg) resulted in anxiolytic-like effects in the elevated plus-maze. The anxiolytic-like effects were also seen after the repeated administration of the HE (100 and 300 mg/kg). Flumazenil (10 mg/kg, i.p.), a GABA_A-benzodiazepine receptor antagonist, blocked the effects of ME (300 mg/kg, p.o.) and HE (600 mg/kg). At higher doses, a sedative effect produced by acute administration of HE (600 mg/kg) or ME (300 mg/kg) was indicated by the potentiation of pentobarbital-induced sleep.

With regard to memory-disrupting effects of anxiolytics, mice were evaluated by measuring the retest step-down latency 24 h after foot-shock in a passive avoidance task. In contrast to diazepam (0.5 mg/kg) or piracetam (200 mg/kg), ME (30, 100 and 300 mg/kg) or HE (100, 300 and 600 mg/kg) did not influence the step-through latency in the acquisition or retention memory tasks. The present results show an anxiolytic profile for HE and ME of Passiflora actinia. There are also indications of an involvement of GABA_A system in this effect.

Phenlypiracetam

Assessing the Validity of Danio rerio as a Model Organism for Nootropic Research: The Effects of Phenylpiracetam on Larval Zebrafish Behavior

Phloretin (apple peel)

Nootropic, neuroprotective and neurotrophic effects of phloretin in scopolamine induced amnesia in mice

Phloretin (PHL), a dihydrochalcone flavonoid usually present in the roots and leaves of apple tree. In vitro study on GT1-7 immortalized hypothalamic neuron s exposed to amyloid beta (25–35), demonstrated that PHL significantly influenced membrane fluidity and potential. PHL also significantly decreased excitotoxicity by restoring the calcium homeostasis in the same. Thus, PHL proves to be a promising therapeutic moiety which should be further screened in the treatment of alzheimer’s disease. The objective of the present study was to evaluate the Nootropic, neuroprotective and neurotrophic roles of PHL in the subacute scopolamine induced amnesia in mice. In this study, mice were pretreated with PHL 2.5 mg/kg, 5 mg/kg, 10 mg/kg and Donepezil (DON) 1 mg/kg intraperitoneally (i.p) for 14 days.

The last 7 days of treatment regimen included daily injection of SCP 1.5 mg/kg to induce Cognitive deficits. Mice were subjected to behavioral analysis. Biochemical estimation of the Brain homogenates for acetylcholine sterase and oxidative stress biomarkers were conducted. Furthermore, immunohistochemical analysis for the Brain derived neurotrophic factor (BDNF) was carried out particularly in the hippocampus. PHL was found to significantly improve the performance of mice in Morris water maze test (P < 0.001) and significantly decreased the acetylcholine sterase activity (P < 0.001) at all doses compared to SCP treated mice. Also, PHL significantly elevated the activity of Antioxidant enzymes viz. superoxide dismutase, catalase, reduced glutathione levels (P < 0.001) and decreased malonaldehyde levels (P < 0.001) in comparison with the SCP group.

Immunohistochemistry revealed that PHL treatment dose dependently improve d BDNF levels in the hippocampus which were found to be significantly depleted (P < 0.001) in the SCP group. Additionally, PHL (10 mg/kg) significantly enhanced the spatial memory formation (P < 0.05) and neurotrophicity (P < 0.001) compared to DON (1 mg/kg). The aforementioned research findings suggested that PHL has Nootropic, neuroprotective and neurotrophic activities in SCP induced memory impaired mice and hence, is a promising therapeutic moiety in the treatment of AD.

Phosphatidylserine (soybean)

Phosphatidylserine and phosphatidylcholine-containing liposomes inhibit amyloid β and interferon-γ-induced microglial activation

There is increasing evidence that microglial activation is one of the major pathogenic factors for alzheimer’s disease (AD) and the inhibition of the inflammatory activation of the microglia thus appears to be neuroprotective and a potentially useful treatment for AD. Phospholipids such as phosphatidylserine (PS) and phosphatidylcholine (PC) have been reported to modulate the immune function of phagocytes. In addition, PS has been reported to be a nootropics that can be used as nonprescription memory or Cognitive enhancers.

We therefore evaluated the effects of liposomes, which comprise both PS and PC (PS/PC liposomes), on the microglial production of tumor necrosis factor-α (TNF-α), nitric oxide (NO), and superoxide (radical dotO2−) induced by amyloid β (Aβ) and interferon-γ (IFN-γ). Pretreatment of microglia with PS/PC liposomes considerably inhibited the TNF-α, NO and radical dotO2− production induced by Aβ/IFN-γ. These results suggest that PS/PC liposomes have both neuroprotective and antioxidative properties through the inhibition of microglial activation, thus supporting the nootropic and antidementia effect of PS.

Phyllanthus Reticulatus Poir

Analyzing Nootropic Effect of Phyllanthus reticulatus Poir. on Cognitive Functions, Brain Antioxidant Enzymes and acetylcholine sterase Activity against Aluminium-Induced Alzheimer’s Model in Rats: Applicable for Controlling the Risk Factors of Alzheimer’s Disease

Oxidative stress is intensely linked with neurodegenerative disorders, especially Alzheimer’s disease (AD). Searching for medicinal plant with the Nootropic activity for controling the development and progression of AD has received extensive consideration. The plant Phyllanthus reticulatus (PR) Poir. is known in Bengali as Panjuli belongs to family Euphorbiaceae. Previous studies have shown the Antioxidant , analgesic, anti-inflammatory, etc. activities of this plant. Therefore, the objective of this study was to examine the Nootropic effect of ethanolic extracts of Phyllanthus reticulatus (EEPR) on Cognitive functions, Brain antioxidatant enzymes and acetylcholine sterase activity in aluminium-induced rats of Cognitive impairment and oxidative stress . The effects of EEPR fruit (i.e., 100 and 200 mg/kg b.w.) were examined for 30 days and its Nootropic effect was determined in aluminium treated Swiss albino male rats by behavioral studies such as Passive Avoidance (PA) test, Rewarded Alternation (RA) test and biochemical studies such as superoxide dismutase (SOD), catalase (CAT), contents of thiobarbituric acid reactive substances (TBARS) and acetylcholine sterase (AChE) activity in rats Brain tissue homogenates. In PA test, administration of EEPR fruit (i.e., 100 and 200 mg/kg, b.w.) significantly (P < 0.05, P < 0.01) increased step-through latency (STL) in rats on 30^th day with respect to disease control group.

The percentage of memory retention (MR) for this test was pointedly (P < 0.05) increased in rats treated with EEPR fruit (i.e., 200 mg/kg b.w.) as compared with disease control group. For RA test, EEPR fruit (i.e., 200 mg/kg b.w.) markedly (P < 0.01) increased the correct responses (CR) in rats on 30^th day related to disease control group. In case of this test the percentage of MR was significantly (P < 0.05, P < 0.01) increased in rats treated with EEPR fruit (i.e., 100 and 200 mg/kg b.w.) with respect to disease control group. Administration of EEPR fruit (i.e., 100 and 200 mg/kg b.w.) considerably (P < 0.05, P < 0.01) increased the level of SOD, CAT and expressively (P < 0.05) decreased TBARS level compared to disease control group. Treatment with EEPR fruit (i.e., 100 and 200 mg/kg b.w.) markedly (P < 0.05, P < 0.01) decreased the level of AChE activity to that of disease control group. The present study shows that EEPR fruit has excellent nootropıc effect on Cognitive performance and Brain Antioxidant markers in aluminium-induced rats of Cognitive impairment and oxidative stress which could be developed in the management of neurodegenerative diseases especially AD.

Piper Longum L

Anti-stress , Nootropic and anticonvulsant potential of fruit extracts of Piper longum L

An aqueous extract [AE] of fruits of Piper longum L. [PL] was investigated for its anti-stress activity in chronic cold restraint stress [(4±0.5 ^oC) for 1h daily for 7 days induced biochemical perturbations in Sprague Dawley rats. The Nootropic potential of PL[AE] was assessed using a passive avoidance model and an elevated plus maze model in Swiss Albino mice. Further, the anticonvulsant activity of the extract was studied against seizures induced by pentylenetetrazole (PTZ), strychnine and 4-aminopyridine in mice. Stimulation of hypothalamus pituitary adrenal (HPA) axis in stress ful condition alters plasma levels of glucose, triglycerides, cholesterol, total proteins and corticosterone.

Pretreatment with the extract (250mg/kg and 500mg/kg p.o.) for 21 days significantly ameliorated the stress -induced biochemical perturbations in rats. PL[AE] treatment significantly increased step-down-latency and shortened the transfer latency in the passive avoidance model and the elevated plus maze model, respectively, suggesting Nootropic potential. Further, treatment of groups with both doses offered protection against PTZ-induced convulsions but failed to protect against strychnine and 4-aminopyridine-induced convulsions in mice. Dimunition in gamma amino butyric acid (GABA) levels in Brain of extract treated mice compared to vehicle treated mice learly indicated involvement of GABAergic mechanisms in the anticonvulsant activity. One-way ANOVA followed by Dunnett’s or Bonferroni test was applied to verify statistical significance.

The present study thus provides scientific support for anti-stress (adaptogenic), Nootropic and anticonvulsant potential of an a aqueous extract of fruits of Piper longum in animal models.

Pramiracetam

Nootropic agents in alzheimer’s disease Symptomatic treatment with pramiracetam

The cognitive-Enhancing effects of pramiracetam in animal models of Learning and memory are characterized by an inverted U-shaped dose-response curve. We evaluated antidementia efficacy of this agent in 10 patients with probable alzheimer’s disease employing a 2-phase, placebo-controlled, enrichment-type trial design. Eight patients evidenced a best dose in the dose-finding phase, but in the subsequent replication phase only two again improve d to a similar degree. PETs with fluorodeoxyglucose obtained in two individuals showed no definite change. Doses up to 4,000 mg pramiracetam are unlikely to confer symptomatic benefit to alzheimer’s disease patients.

Prunus Amygdalus

Nootropic and hypophagic effects following long term intake of almonds(Prunus amygdalus)in rats

Introduction: Over a period of time researchers havebecome more interested in finding out the potential ofvarious foods to maintain the general health and to treatdiseases. Almonds are a very good source of many nutri-ents which may help to sharpen the memory and toreduce cardiovascular risk factors.

Objective: The present study was conducted to evaluatethe Nootropic effects of almonds. Effect of oral intake ofalmond was also monitored on food intake and plasmacholesterol levels. Methods:Rats were given almond paste orally with thehelp of feeding tube for 28 days. memory function in ratswas assessed by Elevated Plus Maze (EPM) and RadialArm Maze (RAM). Brain tryptophan, 5-HT and 5-HIAAwere estimated at the end of the treatment by HPLC-ECmethod.

Results: A significant improve ment in Learning andmemory of almond treated rats compared to controls wasobserved. Almond treated rats also exhibited a significantdecrease in food intake and plasma cholesterol levelswhile the change in growth rate (in terms of percentage)remained comparable between the two groups. Analysisof Brain tryptophan (TRP) monoamines exhibitedenhanced TRP levels and serotonergic turnover in ratBrain following oral intake of almonds.

Conclusion: The findings show that almonds possesssignificant hypophagic and Nootropic effects. Results arediscussed in context of enhanced 5-HT metabolismfollowing almond administration.

Ptychopetalum Olacoides

memory retrieval improve ment by Ptychopetalum olacoides in young and aging mice

Amazonian peoples use traditional remedies prepared with Ptychopetalum olacoides (PO) roots for treating various age-related conditions. This study shows that a single intraperitoneally (i.p.) administration of Ptychopetalum olacoides ethanol extract (POEE, 50 and 100 mg/kg) improve d memory retrieval in step-down inhibitory avoidance (P ≤ 0.05 and P ≤ 0.01, test session latency 102 [19.38–300] and 192 [91.3–300] s, respectively versus control 24.7 [12.9–89.6]), without interfering with acquisition or consolidation in adult (2.5-month-old) mice. Comparable results were obtained with POEE given p.o. at 800 and 1000 mg/kg (P ≤ 0.05 and P ≤ 0.01, 52.7 [19.5–297.2] and 85.7 [44.4–260.4] versus control 20.5 [8–92.6]).

Moreover, memory amelioration was also observed (P ≤ 0.01) in aging (14 months) mice presenting memory deficit (14.95 [10.8–41]) as compared to adult (2.5 months) mice (57 [15.7–141.2]), with the extract given acutely i.p. 100 mg/kg (300 [133.1–300] versus control 14.95 [10.8–41]) or p.o. 800 mg/kg (28.4 [15.1–84.6] versus control 11.5 [7.8–23.3]). Indeed, aging mice treated with POEE (800 mg/kg, p.o.) performed as well as adult mice. Consistently with its traditional use, the data suggest that POEE facilitates memory retrieval. Although the Antioxidant and acetylcholine sterase inhibitory properties previously described for this extract may be of relevance, the molecular mechanism(s) underlying the improve ment in memory retrieval here reported merit further scrutiny.

Pueraria Tuberosa

Nootropic activity of tuber extract of Pueraria tuberosa (roxb)

Nootropic effect of alcoholic (ALE; 50, 75, 100 mg/kg) and aqueous (AQE; 100, 200, 400 mg/kg) extracts of P. tuberosa was evaluated by using Elevated Plus Maze (EPM), scopolamine-induced amnesia (SIA), diazepam-induced amnesia (DIA), clonidine-induced (NA-mediated) hypothermia (CIH), lithium-induced (5-HT mediated) head twitches (LIH) and haloperidol-induced (DA- mediated) catalepsy (HIC) models. Piracetam was used as the standard agent . A significant increase in inflexion ratio (IR) was recorded in EPM, SIA and DIA models.

A significant reversal effect was observed on rectal temperature in CIH model, reduction of head twitches in LIH models. However no significant reduction in catalepsy scores in HIC models were observed with test extracts and standard piracetam. The results indicate that Nootropic activity observed with ALE and AQE of tuber extracts of P. tuberosa could be through improved Learning and memory either by augmenting the noradrenaline (NA) transmission or by interfering with 5-hydroxytryptamine (5-HT) release. Further, the extracts neither facilitated nor blocked release of the dopamine (DA).

Thus ALE and AQE elicited significant Nootropic effect in mice and rats by interacting with cholinergic, GABAnergic, adrenergic and serotonergic systems. Phytoconstituents like flavonoids have been reported for their Nootropic effect and these are present in both ALE and AQE extracts of tubers of P. tuberosa (Roxb) and these active principles may be responsible for Nootropic activity.

L-Pyroglutamate

Investigations on the binding properties of the Nootropic agent pyroglutamic acid

The two stereoisomers of pyroglutamic acid (PCA), a Nootropic or cognition –Enhancing agent, and classic reference compounds were investigated for their ability to interact with 27 neurotransmitter receptors and agent binding sites prepared from selected areas of the central nervous system and labelled with high affinity and selectivity with specific radioligands. L-PCA significantly interacted with the rat foreBrain excitatory amino acid receptors labelled with 3H-L-glutamic acid. The IC50 of L-PCA was 28.11 microM, that of cold L-glutamic acid was 1.68 microM. The corresponding figure for L-aspartic acid was 16.95 microM. The indirect Hill plot gave coefficients of 0.48, 1.08 and 0.75 for L-PCA, L-glutamic and L-aspartic acids, respectively.

Only very high concentration s (10(-4) M) of L-PCA were able to slightly antagonize the specific binding of 3H-clonidine to alpha 2-adrenergic receptors, of 3H-dihydroalprenolol to beta 1- and beta 2-adrenergic receptors of the heart and of the lung and of 3H-diazepam to benzodiazepine receptors. The D-isomer of PCA was practically as active as the L-isomer on these receptors. Finally, L-PCA (10(-5) to 10(-4) M) was unable to antagonize the specific binding of all the other radioligands to their respective receptors and binding sites. D-PCA did not significantly interact with excitatory amino acid receptors or with any of the other sites studied here.

Radix Polygalae

Nootropic Medicine Radix Polygalae and Its Active Constituent Onjisaponin B Reduce β-Amyloid Production and improve Cognitive Impairments

Decline of Cognitive function is the hallmark of Alzheimer’s disease (AD), regardless of the pathological mechanism. Traditional Chinese medicine has been used to combat Cognitive impairments and has been shown to improve Learning and memory. Radix Polygalae (RAPO) is a typical and widely used herbal medicine. In this study, we aimed to follow the β-amyloid (Aβ) reduction activity to identify active constituent(s) of RAPO. We found that Onjisaponin B of RAPO functioned as RAPO to suppress Aβ production without direct inhibition of β-site amyloid precursor protein cleaving enzyme 1 (BACE1) and γ-secretase activities. Our mechanistic study showed that Onjisaponin B promoted the degradation of amyloid precursor protein (APP).

Further, oral administration of Onjisaponin B ameliorated Aβ pathology and behavioral defects in APP/PS1 mice. Taken together, our results indicate that Onjisaponin B is effective against AD, providing a new therapeutic agent for further agent discovery.

Red Ginseng Ginsenoside Rg1 Rb1

Effects of red ginseng saponins and Nootropic agents on impaired acquisition of ethanol-treated rats in passive avoidance performance

Effects of single and repeated administration of red ginseng total saponins (ROTS) and Nootropic agents were examined on impairment of acquisition induced by single oral administration of 3 g/kg ethanol (EtOH) in a step through test. The inhibitory effect of EtOH on acquisition was significantly reduced following single or repeated RGTS administration. The Nootropic agents , piracetam and N-methyl-d-glucamine, given orally significantly reduced impairment of acquisition induced by EtOH.

On the other hand, the inhibitory effect of repeated RGTS on the EtOH-induced amnesia was blocked by the pretreatment of α-methyl-ρ-tyrosine (α-MT), an inhibitor of catecholamine synthesis, in a dose-dependent manner but not ρ-chlorophenylalanine (PCPA), an inhibitor of serotonin synthesis, whereas the inhibitory effect of repeated N-methyl-d-glucamine on the EtOH-induced amnesia was blocked neither by α-MT nor PCPA. These results suggest that repeated RGTS and N-methyl-d-glucamine ameliorate the impairing effect of EtOH on acquisition, and the effect of RGTS on EtOH-induced amnesia is dependent on the catecholaminergic but not serotonergic neuron al activity, while RGTS and N-methyl-d-glucamine seem to have a different mechanism on EtOH-induced amnesia.

Reishi mushroom Polysaccharide

Hypotensive and neurometabolic effects of intragastric Reishi (Ganoderma lucidum) administration in hypertensive ISIAH rat strain

Purpose: One possible candidate is extract from the mushroom Reishi (Ganoderma lucidum), which is used in the treatment and prevention of many chronic diseases.

Study design and methods: To study the effectiveness of Reishi, which grows in the Altai Mountains, as an antihypertensive agent, we intragastrically administered Reishi water extract to adult male hypertensive ISIAH (inherited stress -induced arterial hypertension) rats.

Results: After seven weeks, Reishi therapy reduced blood pressure in experimental animals at a level comparable to that of losartan. Unlike losartan, intragastric Reishi introduction significantly increases Brain Blood Flow from ScienceDirect’s AI-generated Topic Pages cerebral blood flow and affects cerebral cortex metabolic patterns, shifting the balance of inhibitory and excitatory neurotransmitter from ScienceDirect’s AI-generated Topic Pages” 

Salidroside (Rhodiola Rosea)

Rhodiola Rosea: Nootropic Powerhouse?

Quality evidence has been mounting for Rhodiola Rosea’s efficacy as an adaptogen and an all natural Nootropic. It is being used by an increasing number of people to help them cope with heavy work loads, alleviate headaches, improve their concentration , reduce anxiety , enhance their memory , regulate their appetites (too little or too much), bolster their immune systems, and reduce irritability. As an herb that helps the body better cope with stress , none of these benefits should be surprising. Better yet, a sizeable chunk of the claims about Rhodiola have been subject to double-blind studies – meaning there is solid evidence to support many of the claims about it.

Rhodiola is an herbal Nootropic, a natural Brain booster. Subjects in the verum group made far fewer errors in proofreading tasks than the control or those given Siberian ginseng (Rathee, 2008).

Rice Bran Oil

Nootropic and anti-stress effects of rice bran oil in male rats

Rice bran oil (RBO) is an important product of rice bran. It is considered to be one of the most important nutritious oil due to its favorable fatty acid composition and unique composition of naturally occurring biologically active Antioxidant compounds. This study was designed to monitor the effects of oral intake of RBO on stress response in rats. RBO was extracted using hexane. Rats were divided into Control and test (RBO-treated). RBO-treated rats were given 0.2 ml/day RBO for 6 weeks. Food intake and body weight changes were monitored weekly. After 6 weeks open field activity and Morris Water Maze (MWM) test were performed. Results showed that weekly cumulative food intake but not body weight were lower in RBO-treated rats during 1st to 5th week of treatment, which were normalized at the end of treatment. Exploratory activity of RBO-treated rats in an open field was increased.

Spatial memory in Morris water maze was enhanced in RBO-treated than control rats. An episode of 2 h restraint stress decreased the 24 h food intake of both control and RBO-treated animals. Behavioral deficits were lower in RBO-treated rats. Exposure of 2 h restraint stress increased Brain serotonin (5-hydroxytryptamine: 5-HT) metabolism. These increases were lower in RBO-treated restrained than their respective control animals. Serotonergic neurotransmitter mechanism is implicated in stress .

The findings of the study show beneficial effects of RBO in Learning and memory functions. Moreover, the study also highlights the attenuating effect of RBO on stress induced behavioral and neurochemical effects in rats.

Rubia Cordifolia Linn.

Antihyperglycemic, antistress and Nootropic activity of roots of Rubia cordifolia Linn

Effect of alcoholic extract of roots of Rubia cordifolia was studied on elevated blood glucose level in alloxan treated animals. The extract reduced the blood sugar level raised by alloxan. Effect of alcoholic extract was also investigated on cold restraint induced stress and on scopolamine-induced memory impairment. Alcoholic extract enhanced Brain γ-amino-n-butyric acid (GABA) levels and decreased Brain dopamine and plasma corticosterone levels.

Acidity and ulcers caused due to cold restraint stress were inhibited by alcoholic extract. Animals treated with alcoholic extract spent more time in open arm in elevated plus maze model. It also antagonized scopolamine induced Learning and memory impairment. Baclofen induced catatonia was potentiated by alcoholic extract.

Sarsasapogenin-AA13

Astrocytes mediated the Nootropic and neurotrophic effects of Sarsasapogenin-AA13 via upregulating brain-derived neurotrophic factor

Rhizoma Anemarrhena, a widely used traditional Chinese medicine, has previously been shown to have neuroprotective effect. Sarsasapogenin-AA13 (AA13) is a novel synthetic derivative of Sarsasapogenin, which is extracted from Rhizoma Anemarrhena. The aim of this study is to investigate the Nootropic and neurotrophic effects of AA13 and underlying mechanisms. In vitro, cell viability of rat primary astrocytes treated with AA13 and neurons cultured with conditioned medium of AA13-treated rat primary astrocytes was tested by MTT assays.

In vivo, a pharmacological model of Cognitive impairment induced by scopolamine was employed and spatial memory of the mice was assessed by Morris water maze. This study found that AA13 increased cell viability of primary astrocytes and AA13-treated astrocyte-conditioned medium enhanced the survival rate of primary neuron s. Interestingly, AA13 markedly enhanced the level of BDNF in astrocytes. Furthermore, AA13 (6 mg/kg) improved the Cognitive deficits in animal models (p<0.05) and BDNF and PSD95 levels were increased in brain.

Therefore, we hypothesize that AA13 exerts Nootropic and neurotrophic activities through astrocytes mediated upregulation of BDNF secretion. The results suggest that AA13 could be a potential compound for Cognitive impairment after further research.

Shankhpushpi

Nootropic, anxiolytic and CNS-depressant studies on different plant sources

Sida Cordifolia Alkaloids

Evaluation of Nootropic activity of Sida cordifolia in mice

Introduction: In the present study, Nootropic effect of aqueous and hydroethanolic extracts of Sida cordifolia (AESC and EESC, respectively) was investigated in mice using transfer latency (TL) and step-down latency (SDL) tests. S. cordifolia is a well-known Ayurvedic plant which has been administered anciently for the various nervous disorders including loss of memory .

Materials and Methods: Varying doses (50, 100, 250 mg/kg; p.o.) of AESC and EESC were administered along with standard agent (donepezil; 5 mg/kg) for 15 successive days to different groups. They were subjected TL and SDL tests on the 16th day.

Results: EESC dose dependently increased the SDL and decreased the TL in mice as compared to control group, and this effect was comparable to the standard agent . No significant effect on TL and SDL was observed following varying dose treatment of AESC.

Conclusion: These findings suggest the Nootropic effect of EESC and predict its scope in the possible treatment of diseases associated with memory dysfunctions such as Alzheimer’s disease.

Slimaluma

Evaluation of Nootropic activity of Slimaluma, an enriched phytochemical composition of Caralluma fimbriata in mice

In today’s life of stress and strain, the need of agent called Nootropic having neuroprotective and neuropharmacological activity, Enhancing Learning and memory functions of Brain has directed the interest of scientific community to validate the claims made about herbs in the official books of Ayurveda [1, 2]. The Caralluma fimbriata (CF) family Asclepiadaceae is an edible succulent that is claimed to promote Learning and memory but not documented so far[3] hence we have undertaken the evaluation of Nootropic activity of Slimaluma (SL), branded product and an enriched phytochemical composition developed from CF in mice using four different paradigms.

Each model had six groups consisting of six male albino mice each, including vehicle treated control, reference standard Piracetam and four different doses (100, 250, 500 and 1000mg/kg) of SL. The mice were pretreated as per the standard protocol [1, 4, 5, 6] and the data obtained was analysed by using ANOVA followed by the Dunnetts test.

The SL 250, 500mg/kg (P&lt;0.05) and 1000mg/kg (P&lt;0.01) significantly increased the discrimination index Indicating an improve ment in nonspiatal memory with the characteristic of episodic memory [7]. In Morris water maze, all the doses were equally significant in reducing the escape latency while SL 500 and 1000mg/kg significantly reduced the distance traveled and thereby reported its effectiveness towards the memory based upon spatial navigation [6]. The SL-250, 500 and 1000mg/kg were significant in reducing the time spent in open arm and thereby exhibited anxiolytic activity [4]. In radial arm maze, none of the dose was significant, which may be due to the appetite suppression [3]. To conclude, SL significantly facilitated spatial and non-spatial memory functions and exhibited anxiolytic action. Hence may be used to treat the related disorders of Learning and memory and to relive anxiety without affecting the Cognitive function.

Sulbutiamine

The role of the synthetic B1 Vitamin sulbutiamine on health – a review

Sulbutiamine is a thiamine derivative developed in Japan in the ​mid-60 ́s​ as a beriberi treatment agent . Since then, different potential applications have been described. For instance, there is some evidence that sulbutiamine can have anti-fatigue, Nootropic and Antioxidant effects, which led to its use as a sport supplement (although some authors argue it is actually a masking doping strategy). Moreover, this molecule has been proposed as a possible treatment for some microsporidial infections​ ​and even for certain types of cancer​. ​

Despite these potential effects, sulbutiamine is still a relatively unknown molecule, which justifies the present review, where we discuss its history and the existing literature on its health applications. We conclude that there is a great potential for sulbutiamine use, well beyond its first described function (to increase thiamine tissue concentration ). Indeed, new mechanisms of action have been found, mainly associated with its derivatives.

Nevertheless, and although the research on sulbutiamine started 50 years ago, only a limited number of studies were conducted during this time frame. As so, methodological concerns need to be addressed and new studies are necessary, especially randomized controlled trials. Only then, will the full potential of this versatile molecule be identified.

Sunifiram

DM235 (sunifiram): a novel Nootropic with potential as a Cognitive enhancer

Tabernaemontana

The effects of Tabernaemontana divaricata root extract on amyloid β-peptide 25–35 peptides induced Cognitive deficits in mice

Ethnophamacological relevance:

Taxillus Tomentosus

Screening of Taxillus Tomentosus Ethanolic Extract for Nootropic and Antistress Activity in Rats

In recent years, attempts have been made to develop agents for treatment of dementia and attention deficit disorders to improve memory and learning. Some Nootropic agents (eg: Piracetam) are widely used but the resulting chemo phobia associated with them and other similar agents has made their use limited. So it is worthwhile to explore medicines from the traditional system in the treatment of these Cognitive disorders.stress is a broad, ambiguous, and often poorly understood concept. In its most simplified Sense, stress is what one feels when life’s demands exceed one’s ability to meet those demands. The objective of the study is to study the effect of ethanolic extract of Taxillus tomentosus for Nootropic and antistress activity in experimental animals.

It has been documented in traditional system that Taxillus tomentosusplant belonging to family loranthaceaeeffective in nervine disorders, acts as a nervine tonic. In a survey of ethnobotanical literature, numerous plant preparations have been used in the treatment of age related Cognitive disorders in human beings in European countries; so in present study is to evaluate the same on Taxillus tomentosusalone by using various screening models in rats.However, this plant has not been scientifically investigated for the same. Therefore, the present study is designed to evaluate effect of whole plant extract on Learning and memory.

Thespesia Populnea

Pharmacological actions of Thespesia populnea relevant to alzheimer’s disease

Thespesia populnea (Malvaceae) is a large tree found in the tropical regions and coastal forests of India. Various parts of T. populnea are found to possess useful medicinal properties, such as antifertility, antibacterial, anti-inflammatory, Antioxidant , purgative and hepatoprotective activity. The present study was undertaken to investigate the effects of T. populnea bark on Cognitive functions, total cholesterol levels and cholinesterase activity in mice. A total of 312 mice divided into 52 different groups were employed in the present investigation.

The ethanolic extract of T. populnea (TPE) was administered orally in three doses (100, 200 and 400 mg/kg) for 7 successive days to different groups of young and aged mice. The Learning and memory parameters were assessed using elevated plus maze and passive avoidance apparatus. TPE (200 and 400 mg/kg, p.o.) showed significant improve ment in memory of young and aged mice. TPE also reversed the amnesia induced by scopolamine (0.4 mg/kg, i.p.) and diazepam (1 mg/kg, i.p.). Furthermore, TPE reduced significantly the central (brain) cholinesterase activity in mice. TPE exhibited a remarkable cholesterol lowering property comparable to simvastatin (a standard agent ) in the present study.

Furthermore, we observed that, T. populnea bark possessed a powerful memory Enhancing activity in mice. Since diminished cholinergic Receptor Stimulating Agent from ScienceDirect’s AI-generated Topic Pages cholinergic transmission and increased cholesterol levels appear to be responsible for development of amyloid plaques and dementia from ScienceDirect’s AI-generated Topic Pages dementia in Alzheimer patients, TPE may prove to be a useful medicine on account of its multifarious beneficial effects, such as memory improving property, cholesterol lowering, acetylcholine sterase Inhibitor from ScienceDirect’s AI-generated Topic Pages anticholinesterase and anti-inflammatory activity. Therefore, T. populnea bark appears to be a promising candidate for improving memory and it would be worthwhile to explore the potential of this plant in the management of Alzheimer patients.

Tiliacora Racemosa Colebr

Evaluation of antibacterial, Antioxidant and Nootropic activities of Tiliacora racemosa Colebr. leaves: In vitro and in vivo approach

Tinospora Cordifolia

Nootropic Activity of Saponins obtained from Tinospora Cordifolia Stem in Scopolamine induced Amnesia

The present study was designed to evaluatethe Nootropic property of n-butanolic (TBF) fraction of ethanolic extract of Tinospora cordifoliastem which contain saponin. The TBF was administered at 100 mg/kg and 200 mg/kg. The Nootropic effect was evaluated using different models like elevated plus mazein mice to determine the transfer latency on day-2 and Day-9. The passive avoidance test was carried out in mice to evaluate the step down latencyon day-2 and Day-9. The object recognition test carried out to determine the recognition index. Scopolamine(1 mg/kg) was used as amnesic agent and piracetam (250 mg/kg) was used as a standard Nootropic agent.

The results of the present study showed significant increase in transfer latency by TBF on day -2 and Day-9. The TBF also increases step down latency, object recognition index significantly.Scopolamine was used to produce amnesia significantly decreased transfer latency, step down latency, object recognition index.The TBF antagonised the amnesic effects of scopolamine in the above experimental models. Anti-cholinesterase activity of TBF was evaluated by estimation of acetylcholine steras (AchE) concentration in mice Brain after 7-days treatment with TBF. The result showed decreased in AchE concentration indicating involvement of cholinergic system in Nootropic activity of TBF.

Trapa Bispinosa

Nootropic activity of hydroalcoholic extract of Trapa bispinosa

The effect of hydroalcoholic extract of fruits of Trapa bispinosa (TB) was investigated for its Nootropic activity using various experimental paradigms of Learning and memory, viz. transfer latency (TL) on elevated plus-maze, passive avoidance response (PAS) and object recognition test. The investigation reported that TB 500 mg/kg significantly reduced the TL on 2nd and 9th day while TB 250 mg/kg was found effective on 9th day. TB 250 and 500mg/kg significantly increased the step down latency in the PAS at acquisition and retention test. Moreover the TB (250 & 500mg/kg) increased discrimination index in the object recognition test indicating Nootropic activity.

To conclude TBextract showed significant facilitatory effect on aversively motivated Learning and memory in mice as well as improve ment of memory in absence of Cognitive deficit.

Unifiram

The novel Nootropic compound DM232 (UNIFIRAM) ameliorates memory impairment in mice and rats

The favorable pharmacological profile exhibited by piracetam stimulated the synthesis of related compounds potentially endowed with a higher Nootropic potency. The antiamnesic and proCognitive activity of DM232 (unifiram), a new compound structurally related to piracetam, was investigated. Mouse passive avoidance and rat Morris water maze and Social Learning tests were employed. DM232 (0.001–1 mg kg⁻¹ i.p. – 0.01–0.1 1 mg kg⁻¹ p.o.) prevented amnesia induced by scopolamine (1.5 mg kg⁻¹ i.p.), mecamylamine (20 mg kg⁻¹ i.p.), baclofen (2 mg kg⁻¹ i.p.), and clonidine (0.125 mg kg⁻¹ i.p.). Furthermore, The antiamnesic effect of the investigated compound was comparable to that exerted by well‐known Nootropic agents such as piracetam (30–100 mg kg⁻¹ i.p.), aniracetam (100 mg kg⁻¹ p.o.), rolipram (30 mg kg⁻¹ p.o.), and nicotine (5 mg kg⁻¹ i.p). DM232 (0.1 mg kg⁻¹ i.p.) was also able to prevent amnesia induced by scopolamine (0.8 mg kg⁻¹ i.p.) in the rat Morris watermaze test. In the rat social Learning test, DM232 (0.1 mg kg⁻¹ i.p.) injected in adults rats reduced the duration of active exploration of the familiar partner in the second session of the test.

DM232, similarly to piracetam, reduced the duration of hypnosis induced by pentobarbital. At the highest effective doses, the investigated compound did not impair motor coordination (rota rod test), nor modified spontaneous (Animex). These results indicate DM232 (unifiram) as a novel cognition enhancer, strictly related to piracetam‐like compounds, able to ameliorate memory impairment at doses about 1,000 times lower than the most active available Nootropic compounds.

Vateria Indica Vitis vinifera L.

Nootropic and neuroprotective effects of ethanol extract of Vateria indica L bark on scopolamine-induced Cognitive deficit in mice

Purpose: To investigate the neuroprotective and memory-boosting properties of ethanol extract of Vateria indica bark on scopolamine-mediated defects in Learning and memory in young mice.

Methods: The ethanol extract of V. indica bark was prepared via Soxhlet extraction and subjected to qualitative and quantitative phytochemical assessment. The acute toxicity of the extract was also evaluated in mice. Six groups of 3-month-old Swiss albino mice (6 per group) were used: normal control, negative control, piracetam group, 250 mg/kg V. indica extract alone group, 500 mg/kg V. indica extract alone group, 250 mg/kg V. indica extract + scopolamine group and 500 mg/kg V. indica extract + scopolamine group. The mice were pretreated with piracetam (standard Nootropic agent ) or varied doses of the extract for 14 days prior to induction of amnesia. With the exception of normal control group, amnesia induction using scopolamine (3 mg/kg) i.p. on day 14 at 1½ h after the last extract dose. Mice in normal and negative control groups received 0.5 % tragacanth orally at a dose of 10 mL/kg. Cognitive deficit was assessed using elevated plus maze (EPM), step-down avoidance, and Morris water maze (MWM) tests.

Results: Qualitative phytochemical screening of V. indica bark extract showed flavonoid s, phenolics, glycosides, tannins, carbohydrate, saponins and steroids. The total phenol and total flavonoid contents were 580.96 ± 0.95 mg GAE/g extract and 66.89 ± 0.56 mg RE/g extract, respectively. The mice tolerated the extract up to 5000 mg/kg bwt. They all survived during and after the acute toxicity study and no significant changes in appearance or general behavior were noticed. The extract significantly enhanced Learning and memory, and improve d spatial recognition in scopolamine-induced amnesic mice (p < 0.05). acetylcholine sterase (AChE) activity and levels of dopamine and noradrenaline were markedly higher in negative control mice than in normal control, but were significantly reduced after pretreatment with ethanol extract of V. indica bark (p < 0.05). The results of histopathological examination provided evidence in support of the protective effect of the extract on hippocampal and cortical neurons.

Conclusion: Pretreatment with ethanol extract of V. indica bark confers neuroprotection and enhances memory in young amnesic mice. Therefore, the extract of the plant can potentially be develpoed for the management of degenerative Brain conditions.

Vigna Mungo

Nootropic EFFECT OF VIGNA MUNGO(L.) HEPPER SEEDS EXTRACT IN SCOPOLAMINE INDUCED AMNESIC RATS

Aim of the Study: Vigna mungo (L.) Hepper seeds extract consisted of flavonoids, phenolic compounds and Vitamin C etc. have important Antioxidant functions that can protect against oxidative stress in the Brain and damage associated with neurodegenerative disorders. The present study was investigated the protective effect of ethanolic extract of Vignamungo (L.) Hepper seeds on Learning and memory functions in scopolamine induced memory deficits rats.

Materials and Methods: In scopolamine induced Cognitive deficit rat model Wistar albino rats of either sex weighing 200-300 g were divided into 8 groups (6 animals per group). Group 1 served as normal control (NC), Group 2 received SCO (2 mg/kg i.p.), Groups 3, 4 and 5 served as per se groups received piracetam (PR) (200 mg/kg i.p.), EVME (200mg/kg p.o.) and EVME (400 mg/kg p.o.), Groups 6, 7 and 8 servedas Cognitive deficit groups which received PR (200 mg/kg i.p), EVME (200 mg/kg p.o.) and EVME (400 mg/kg p.o.) for 28 days respectively followed by SCO (2 mg/kg i.p.). The protective and Cognitive Enhancing effects of EVME on Cognitive deficit rats induced by scopolamine were investigated by assessingthe elevated plus mazeand the Morris water maze test. In order to confirm the underlying mechanisms of memory Enhancing effects of EVME, activities of AChE.

Vinpocetine (Cavinton)

The Nootropic agent Vinpocetine Inhibits Veratridine-Induced [Ca2+]i Increase in Rat hippocampal CA1 Pyramidal Cells

The alkaloid derivative vinpocetine (14-ethoxycarbonyl-(3α,16α-ethyl)-14,15-eburnamine; Cavinton) has a well known beneficial effect on Brain function in hypoxic and ischemic conditions. While it increases CNS blood flow and improves cellular metabolism, relatively little is known about vinpocetine’s underlying molecular mechanisms on the single cell level. Since apoptotic and necrotic cell damage is always preceded by an increase in [Ca²⁺]_i, this study investigated the effect of vinpocetine on [Ca²⁺]_i increases in acute Brain slices. Sodium influx is an early event in the biochemical cascade that takes place during ischemia. The alkaloid veratridine can activate this Na⁺ influx, causing depolarization and increasing [Ca²⁺]_i in the cells.

Therefore, it can be used to simulate an ischemic attack in Brain cells. Using a cooled CCD camera-based ratio imaging system and cell loading with fura 2/AM, the effect of vinpocetine on [Ca²⁺]_i changes in single pyramidal neurons in the vulnerable CA1 region of rat hippocampal slices was investigated. Preperfusion and continuous administration of vinpocetine (10 μM) significantly inhibited the elevation in [Ca²⁺]_i induced by veratridine (10 μM). When the agent was administered after veratridine, it could accelerate the recovery of cellular calcium levels. Piracetam, another Nootropic used in clinical practice, could attenuate the elevation of [Ca²⁺]_i only at a high, 1 mM, concentration. We have concluded that vinpocetine, at a pharmacologically relevant concentration, can decrease pathologically high [Ca²⁺]_i levels in individual rat hippocampal CA1 pyramidal neurons; this effect might contribute to the neuroprotective property of the agent.

Vitis vinifera L.

Adaptogenic and Nootropic activities of aqueous extract of Vitis vinifera (grape seed): an experimental study in rat model

Yashtimadhu Choorna

Comparative assessment of Nootropicactivity of Yashtimadhu Choorna and Yashtimadhu Kshirapaka in young and aged mice

Yashtimadhu is one of the “Medhya Rasayan” agents advocated to achieve desired effect on Cognitive functions especially when given with Cow milk. In present study two dosage forms of Yashtimadhu i.e. Choorna [Powder] and Kshirapaka[Medicated Milk] were prepared as per standard guidelines given in ayurvedic classical text and were analysed with API parameters. Therefore, it can be saidthat a preparation method of both formulations is developed and validated with physic-chemical analysis. In extension, comparative assessment of Nootropic activity of both formulations was done by usingtwo animal models, viz. Diazepam induced amnesia using elevated plus maze [EPM] in young mice and spatial learning and memory in radial arm maze [RAM] in aged mice. In EPM test and RAM test agent administration was done daily for 15 and 7 days respectively.

Study finding suggests that Yashtimadhu Kshirpaka at therapeutic dose exhibited anti-amnesic effect and learning-memory enhancement action in EPM test and RAM test (P<0. 001). The result also specified comparative significant effect of Yashtimadhu Choorna with cow milk on animal’s cognition than Yashtimadhu Choorna withwater. Thus it can be concluded that addition of cow milk as Anupana to Yashtimadhu or processing of Yashtimadhu Choorna with cow milk in ‘’Kshirapaka formulation’’ has potential to maintain healthy Cognitive process in animals’. Hence both potent formulations can be employed as Nootropic agents to maintain human cognition in balanced state or can be utilized as adjuvant to conventional treatment.

Gingerol (Zingiber Officinale)

ZINGIBER OFFICINALE: EVALUATION OF ITS Nootropic EFFECT IN MICE

Dementia is one of the age-related Mental problems, and a characteristic symptom of Alzheimer’s disease. Nootropic agents and cholinesterase inhibitors like donepezil^® are clinically used in situations where there is organic disorder in Learning abilities and for improving memory, Mood and behavior, but the resulting side-effects associated with these agents have made their utility limited. Ayurveda emphasizes use of herbs, nutraceuticals or life-style changes for controlling age related neurodegenerative disorders. The present study was undertaken to assess the potential of an ayurvedic rasayana (rejuvenator) agent Zingiber officinale Roscoe as a memory enhancer . Elevated plus maze and passive avoidance paradigm were employed to evaluate Learning and memory parameters. Z. officinale extract (50 and 100 mg/kg, p.o.) were administered for 8 successive days to both young and aged mice.

The dose of 100 mg/kg of Z. officinale extract significantly improved Learning and memory in young mice and also reversed the amnesia induced by diazepam (1 mg/kg, i.p.), and scopolamine (0.4 mg/kg, i.p.). Furthermore, it also reversed aging induced amnesia due to natural aging of mice. Z. officinale significantly increased whole Brain acetyl cholinesterase inhibition activity. Hence, Z. officinale might prove to be a useful memory restorative agent in the treatment of dementia seen in the elderly. The underlying mechanism of its action may be attributed to its Antioxidant and acetyl cholinesterase inhibition property.